Vorobjova T, Grünberg H, Oona M, Maaroos H I, Nilsson I, Wadström T, Covacci A, Uibo R
Department of Immunology, University of Tartu, Estonia.
Eur J Gastroenterol Hepatol. 2000 Jan;12(1):97-101. doi: 10.1097/00042737-200012010-00018.
To evaluate Helicobacter pylori and CagA seropositivity in a non-selected group of schoolchildren in southern Estonia, with reference to previous studies where high seroprevalence to H. pylori (87%) and anti-CagA positivity (63%) in an adult population from the same region were found.
A total of 421 schoolchildren selected haphazardly from a random population (n = 1018, ages 9, 12 or 15 years) and living in urban or rural areas.
H. pylori status was determined by evaluation of IgG antibodies against cell surface proteins of H. pylori, strain CCUG 17874, using standard ELISA. Anti-CagA IgGs were determined by ELISA using a recombinant fragment of CagA (CCUG 17874) as solid-phase antigen. Absorbance values > 0.3 (405 nm) were taken as a CagA-positive result based on a study of 25 sera from H. pylori-negative children.
Of the 421 subjects, 235 (56%) were H. pylori-ELISA positive, and 109 out of the 235 (46%) were anti-CagA positive. Neither H. pylori nor CagA positivity were significantly different in girls and boys, or in children aged 9, 12 or 15 years. The H. pylori prevalence rate (118/181, 65%) as well as CagA positivity (64/181, 35%) in rural areas were higher compared with those in towns (117/240, 49% and 54/240, 22%, respectively; P = 0.001 and P = 0.005).
Of schoolchildren living in southern Estonia, 56% were seropositive to H. pylori. Half of them had anti-CagA antibodies. Schoolchildren living in rural areas were infected significantly more often with CagA-seropositive strains compared with those living in towns.
参照之前在爱沙尼亚南部同一地区成年人群中幽门螺杆菌血清阳性率较高（87%）和抗CagA阳性率较高（63%）的研究，评估爱沙尼亚南部一组未经过挑选的学童中幽门螺杆菌和CagA血清阳性情况。
从随机人群（n = 1018，年龄9、12或15岁）中随机抽取421名学童，他们生活在城市或农村地区。
采用标准酶联免疫吸附测定法（ELISA），通过评估针对幽门螺杆菌菌株CCUG 17874细胞表面蛋白的IgG抗体来确定幽门螺杆菌感染状况。使用CagA重组片段（CCUG 17874）作为固相抗原，通过ELISA测定抗CagA IgG。根据对25份幽门螺杆菌阴性儿童血清的研究，吸光度值> 0.3（405 nm）被视为CagA阳性结果。
在421名受试者中，235名（56%）幽门螺杆菌ELISA检测呈阳性，其中109名（46%）抗CagA呈阳性。幽门螺杆菌和CagA阳性在女孩和男孩之间，以及9、12或15岁儿童之间均无显著差异。农村地区的幽门螺杆菌感染率（118/181，65%）以及CagA阳性率（64/181，35%）高于城镇地区（分别为117/240，49%和54/240，22%；P = 0.001和P = 0.005）。
生活在爱沙尼亚南部的学童中，56%幽门螺杆菌血清呈阳性。其中一半有抗CagA抗体。与生活在城镇的学童相比，生活在农村地区的学童感染CagA血清阳性菌株的频率明显更高。
