通过X射线光电子能谱法测定RGP镜片上的蛋白质沉积物。

Determination of protein deposits on RGP lenses by X-ray photoelectron spectroscopy.

作者信息

Ichijima H, Kawai T, Yamamoto K, Cavanagh H D

机构信息

Research and Development Department, Menicon Co., Ltd., Kasugai, Aichi, Japan.

出版信息

CLAO J. 2000 Jan;26(1):18-20.

PMID:10656304

Abstract

PURPOSE

To establish a novel and objective screening method for evaluating the cleaning efficacy of contact lens care solutions for removal of protein deposits on single rigid gas permeable (RGP) lenses.

METHODS

New and unworn RGP lenses containing no nitrogen atoms were incubated in a standard tear protein test solution. Protein deposition on lenses was determined by X-ray photoelectron spectroscopy (XPS) before and after standardized cleaning with commercially available care solutions (daily cleaners A, B) and a wetting/soaking solution or with 0.1w/v% sodium dodecyl sulfate (SDS) as a control. Cleaning efficacy was calculated based on the percentage of nitrogen atoms present in the total elements of the measured XPS scan spectrum.

RESULTS

Nitrogen atoms originating from residual protein deposits on RGP lenses after cleaning were determined by XPS. The cleaning efficacy of the wetting/soaking solution was lower (74%) than that of daily cleaners A and B (95%) or SDS (96%) controls.

CONCLUSIONS

XPS determination of residual protein deposits is a novel and direct evaluation method for determining the cleaning efficacy of RGP lens care solutions for single lenses.

摘要

目的

建立一种新颖且客观的筛选方法，用于评估隐形眼镜护理液对去除单个硬性透气性（RGP）镜片上蛋白质沉积物的清洁效果。

方法

将不含氮原子的全新未佩戴RGP镜片置于标准泪液蛋白质测试溶液中孵育。在用市售护理液（日常清洁剂A、B）、润眼/浸泡液或0.1w/v%十二烷基硫酸钠（SDS）作为对照进行标准化清洁前后，通过X射线光电子能谱（XPS）测定镜片上的蛋白质沉积情况。基于所测XPS扫描光谱总元素中存在的氮原子百分比计算清洁效果。