Hu J, Jara F, Thakrar J, Shih K
Pilkington Barnes Hind, Sunnyvale, CA 94086, USA.
CLAO J. 1995 Jul;21(3):154-5.
We developed a nondestructive, quantitative method for evaluating the cleaning efficacy of rigid gas permeable contact lens care systems. Simulated tears prepared with lysozyme, albumin, and calcium ions were applied to rigid gas permeable lenses as a protein soiling model, and a mixture of grease and unsaturated fat was applied as a lipid soiling model. The ComfortCare system (Pilkington Barnes Hind) and the Boston Cleaner and Boston Conditioning Solution (Polymer Technology) were evaluated for their efficacy in removing protein deposits; water was used as a control. The ComfortCare system and the Boston Advance Cleaner and Boston Advance Conditioning Solution were evaluated for their effectiveness in removing lipid deposits; saline was used as a control. Protein deposit level was detected by a densitometer at the absorbance of 280 nm. The lipid deposit level was evaluated by the absorbance of the fluorescence at 430 nm using the same densitometer with a fluorescence accessory. The two test systems demonstrated a significantly higher cleaning efficacy than the control for both soiling models. The methods provide quantitative measurements and therefore can be analyzed statistically for screening or comparison purposes.
我们开发了一种用于评估硬性透气性隐形眼镜护理系统清洁效果的无损定量方法。用溶菌酶、白蛋白和钙离子制备的模拟泪液作为蛋白质污染模型应用于硬性透气性隐形眼镜，用油脂和不饱和脂肪的混合物作为脂质污染模型。评估了ComfortCare系统（皮尔金顿·巴恩斯·欣德公司）以及波士顿清洁液和波士顿护理液（聚合物技术公司）去除蛋白质沉积物的效果；用水作为对照。评估了ComfortCare系统以及波士顿高级清洁液和波士顿高级护理液去除脂质沉积物的效果；用生理盐水作为对照。通过密度计在280nm吸光度下检测蛋白质沉积物水平。使用配备荧光附件的同一台密度计，通过430nm荧光的吸光度评估脂质沉积物水平。对于两种污染模型，这两种测试系统均显示出比对照显著更高的清洁效果。这些方法提供了定量测量结果，因此可用于统计分析以进行筛选或比较。
