Yung L Y, Lim F, Khan M M, Kunapuli S P, Rick L, Colman R W, Cooper S L
Department of Chemical Engineering, University of Delaware, Newark 19716, USA.
Biomaterials. 2000 Feb;21(4):405-14. doi: 10.1016/s0142-9612(99)00203-3.
Adsorbed proteins on biomaterial surfaces determine whether cells adhere, but rheological variables are also critical. Neutrophil adhesion under well-defined radial flow conditions was studied on glass preadsorbed with plasma proteins or plasma protein domain fragments. Fibrinogen, low-molecular-weight kininogen (LK), high-molecular-weight kininogen (HK), cleaved HK (HKa), and recombinant HK domains 3 and 5 (D3 and D5H) were used. The number of adherent cells on the HK and HKa surfaces was less than 10% that found on the fibrinogen absorbed surface. The degree of spreading was minimal and detachment of adherent neutrophils was observed. HK and HKa contain binding sites for both anionic surfaces and neutrophils in the same domain (D5H). When adsorbed to surfaces, HK and HKa did not have the neutrophil binding sites available and therefore exhibited an anti-adhesive effect. Although D5H contains anionic surface binding sites, its small molecular size required a higher number of adsorbed molecules to cover the surface before a significant decrease in cell adhesion was observed. Since LK and D3 do not possess specific anionic surface binding sites, the adsorption of these proteins on glass was very low compared to HK and HKa. Thus, extensive cell adhesion and spreading were observed on the surfaces partially covered with preadsorbed LK and D3.
生物材料表面吸附的蛋白质决定细胞是否黏附，但流变学变量也很关键。在预先吸附了血浆蛋白或血浆蛋白结构域片段的玻璃上，研究了在明确的径向流条件下中性粒细胞的黏附情况。使用了纤维蛋白原、低分子量激肽原（LK）、高分子量激肽原（HK）、裂解的HK（HKa）以及重组HK结构域3和5（D3和D5H）。HK和HKa表面上的黏附细胞数量不到纤维蛋白原吸附表面上的10%。铺展程度极小，并且观察到黏附的中性粒细胞发生脱离。HK和HKa在同一结构域（D5H）中同时含有阴离子表面和中性粒细胞的结合位点。当吸附到表面时，HK和HKa没有可用的中性粒细胞结合位点，因此表现出抗黏附作用。尽管D5H含有阴离子表面结合位点，但其小分子尺寸需要更多数量的吸附分子来覆盖表面，才会观察到细胞黏附显著减少。由于LK和D3不具备特定的阴离子表面结合位点，与HK和HKa相比，这些蛋白质在玻璃上的吸附非常低。因此，在部分覆盖有预先吸附的LK和D3的表面上观察到广泛的细胞黏附和铺展。
