Department of Chemical and Biomolecular Engineering, Ohio State University, Columbus, Ohio 43210, USA.
Tissue Eng Part A. 2013 May;19(9-10):1113-21. doi: 10.1089/ten.TEA.2011.0653. Epub 2013 Jan 30.
The initial adhesion of human umbilical vein endothelial cells (HUVECs), cord blood endothelial colony-forming cells (ECFCs), and human blood outgrowth endothelial cells (HBOECs) was studied under radial flow conditions. The surface of a variable shear-rate device was either coated with polymer films or covered by synthetic fibers. Spin-coating was applied to produce smooth polymer films, while fibrous scaffolds were generated by electrospinning. The polymer was composed of hexyl methacrylate, methyl methacrylate, poly(ethylene glycol) methacrylate (PEGMA), and CGRGDS peptide. The peptide was incorporated into the polymer system by coupling to an acrylate-PEG-N-hydroxysuccinimide comonomer. A shear-rate-dependent increase of the attached cells with time was observed with all cell types. The adhesion of ECs increased on RGD-linked polymer surfaces compared to polymers without adhesive peptides. The number of attached ECFCs and HBOECs are significantly higher than that of HUVECs within the entire shear-rate range and surfaces examined, especially on RGD-linked polymers at low shear rates. Their superior adhesion ability of endothelial progenitor cells under flow conditions suggests they are a promising source for in vivo seeding of vascular grafts and shows the potential to be used for self-endothelialized implants.
研究了在径向流条件下，人脐静脉内皮细胞（HUVEC）、脐血内皮祖细胞（ECFC）和人血衍生的血管内皮细胞（HBOEC）的初始黏附。采用可变剪切率装置的表面涂覆聚合物膜或覆盖合成纤维。旋涂法用于制备光滑的聚合物膜，而电纺丝法用于生成纤维状支架。聚合物由己基甲基丙烯酸酯、甲基丙烯酸甲酯、聚乙二醇甲基丙烯酸酯（PEGMA）和 CGRGDS 肽组成。肽通过与丙烯酰基-PEG-N-羟基琥珀酰亚胺共聚单体偶联到聚合物系统中。观察到所有细胞类型的黏附细胞随时间呈剪切率依赖性增加。与不含粘附肽的聚合物相比，EC 细胞在与 RGD 相连的聚合物表面上的黏附增加。在所检查的整个剪切率范围和表面上，附着的 ECFC 和 HBOEC 的数量明显高于 HUVEC 的数量，尤其是在低剪切率下与 RGD 相连的聚合物上。它们在流动条件下的内皮祖细胞的优越黏附能力表明，它们是血管移植物体内播种的有前途的来源，并显示出用于自内皮化植入物的潜力。
