Osawa T
Department of Applied Biological Sciences, Nagoya University, Chikusa, Japan.
Mech Ageing Dev. 1999 Nov;111(2-3):133-9. doi: 10.1016/s0047-6374(99)00069-x.
The oxidative break down of the membrane polyunsaturated fatty acids is known to be accompanied by the formation of a complex mixture of lipidhydroperoxides and secondary products. These compounds are highly reactive and are capable of rapid reaction with cellular nucleophiles such as phospholipids and proteins, and it was found that these reaction products are candidates as impotant biomarkers to evaluate antioxidative activity of dietary antioxidants. The author has been involved in developing immunochemical detection methods for oxidative stress by application of polyclonal and monoclonal antibodies. From the hypothesis that endogenous antioxidants in plants must play an important role for antioxidative defense systems from oxidative stress, an intensive search for novel type of natural antioxidants has been carried out from numerous plant materials, including those used as foods, and we have isolated and identified a number of lipid-soluble and water-soluble dietary antioxidants from crop seeds, sesame seeds and some spices. In this paper, the recent progress of research on functions of dietary antioxidants is reviewed.
已知膜多不饱和脂肪酸的氧化分解伴随着脂质氢过氧化物和次级产物的复杂混合物的形成。这些化合物具有高反应性，能够与细胞亲核试剂如磷脂和蛋白质快速反应，并且发现这些反应产物作为评估膳食抗氧化剂抗氧化活性的重要生物标志物的候选物。作者一直致力于通过应用多克隆和单克隆抗体开发氧化应激的免疫化学检测方法。基于植物中的内源性抗氧化剂必定在抗氧化防御系统抵御氧化应激中发挥重要作用这一假设，已从包括用作食物的材料在内的众多植物材料中进行了新型天然抗氧化剂的深入搜索，并且我们已经从作物种子、芝麻种子和一些香料中分离并鉴定了许多脂溶性和水溶性膳食抗氧化剂。本文综述了膳食抗氧化剂功能的最新研究进展。
