Mohanan P V, Joseph R, Ramesh P, Rathinam K
Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum, India.
J Biomater Appl. 2000 Jan;14(3):224-8. doi: 10.1177/088532820001400302.
Chromosomal aberrations are microscopically visible changes in the chromosome structure. The double-stranded breaks are the ultimate DNA lesions for chromosomal aberrations. The purpose of the present study was to evaluate the induction of chromosomal aberrations by the rubber accelerator zinc mercapto benzo thiazole (ZMBT). The experiment was designed with five groups, each composed of four Swiss albino mice. The first three groups received ZMBT at 1920, 960, and 480 microg/20 g animal. The remaining two groups were the vehicle (cotton seed oil) and positive (methyl methane sulphonate) controls. Animals were given a single dose of test and control samples by IP injection. Colchicine (20 microg/animal) was administered 90 minutes before sacrificing the animals. All the animals were sacrificed at the end of 36 h by cervical dislocation. Bone marrow preparations were made, stained with Giemsa stain, and examined for chromosomal abnormalities. The results indicated a lack of incidence of chromosomal abnormalities in the test and control groups. However, significant chromosomal abnormalities such as gaps, breaks, and translocations were observed in the positive control group. Hence, the study concluded that ZMBT at different concentrations fails to induce structural chromosomal aberrations in bone marrow cells.
染色体畸变是染色体结构在显微镜下可见的变化。双链断裂是导致染色体畸变的最终DNA损伤。本研究的目的是评估橡胶促进剂巯基苯并噻唑锌（ZMBT）对染色体畸变的诱导作用。实验设计了五组，每组由四只瑞士白化小鼠组成。前三组分别给予1920、960和480微克/20克动物体重的ZMBT。其余两组分别为赋形剂（棉籽油）对照组和阳性（甲基磺酸甲酯）对照组。通过腹腔注射给动物单次给予测试和对照样品。在处死动物前90分钟给予秋水仙碱（20微克/只动物）。36小时结束时，通过颈椎脱臼处死所有动物。制备骨髓涂片，用吉姆萨染色，并检查染色体异常情况。结果表明，测试组和对照组均未出现染色体异常情况。然而，在阳性对照组中观察到了明显的染色体异常，如裂隙、断裂和易位。因此，该研究得出结论，不同浓度的ZMBT未能在骨髓细胞中诱导结构性染色体畸变。
