Mohanan P V, Rathinam K
Division of Toxicological Screening of Materials, Sree Chitra Thirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
Vet Hum Toxicol. 1993 Aug;35(4):297-8.
Indigenously developed bone wax was evaluated for cytogenetic effects of a saline extract of the bone wax. Swiss mice were assigned to 5 groups of 6 animals each. Groups I, II, III were given non-lethal doses of 2.5, 5.0 or 10.0 ml bone-wax saline extract/kg body wt and Groups IV and V received sterile 0.9% saline vehicle or cyclophosphamide ip as controls. After 24 h the mice were sacrificed by cervical dislocation. Bone marrow preparations were stained with Giemsa's stain and examined by light microscopy. Abnormalities such as numbers of chromosome gaps, breaks and translocations were noted. The bone-wax saline extract did not induce chromosomal aberrations in the bone marrow of Swiss mice under our laboratory conditions.
对自主研发的骨蜡的骨蜡盐水提取物进行了细胞遗传学效应评估。将瑞士小鼠分为5组，每组6只动物。第I、II、III组分别给予2.5、5.0或10.0 ml/kg体重的非致死剂量骨蜡盐水提取物，第IV组和第V组分别腹腔注射无菌0.9%生理盐水或环磷酰胺作为对照。24小时后，通过颈椎脱臼处死小鼠。骨髓涂片用吉姆萨染色并通过光学显微镜检查。记录染色体间隙、断裂和易位等异常情况。在我们的实验室条件下，骨蜡盐水提取物未在瑞士小鼠骨髓中诱导染色体畸变。
