Takeuchi H, Asai N, Tanabe K, Kozaki T, Fujita M, Sakai T, Okuda A, Naruse N, Yamamoto S, Sameshima T, Heida N, Dobashi K, Baba M
Tsukuba Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan.
J Antibiot (Tokyo). 1999 Nov;52(11):971-82. doi: 10.7164/antibiotics.52.971.
For the purpose of discovering novel agents that inhibit HIV-1 replication at the transcriptional level, we have established cell lines reflecting the HIV-1 long terminal repeat-driven gene expression. Using these cell lines, we have screened approximately 10,000 microorganism products and found that the culture supernatant of Streptomyces sp. Mer-2487 suppresses the HIV-1 Tat-induced gene expression without affecting the basal or tumor necrosis factor-alpha-induced transcription. The purified active component has a unique structure, as shown in Fig. 1. This compound has an inhibitory effect on HIV-1 replication in chronically infected cells as well as acutely infected cells, suggesting that the inhibition occurs at a postintegration step of HIV-1 proviral DNA in the HIV-1 replication cycle.
