Jung H W, Hwang B K
Department of Agricultural Biology, Korea University, Seoul.
Mol Plant Microbe Interact. 2000 Jan;13(1):136-42. doi: 10.1094/MPMI.2000.13.1.136.
Specific cDNAs showing differential expression in bacteria-infected pepper leaves as opposed to healthy leaves were isolated from a pepper cDNA library from hypersensitive response (HR) lesions of leaves infected with an avirulent strain of Xanthomonas campestris pv. vesicatoria. Among a total of 282 cDNA clones tested, 36 individual cDNA genes (13%) hybridized strongly or differentially to the cDNA probes from bacteria-infected leaves. Ten Capsicum Annuum-Induced (CAI) genes encoding putative thionin, lipid transfer protein I and II, osmotin (PR-5), class I chitinase, beta-1,3-glucanase, SAR 8.2, stellacyanin, leucine-rich repeat protein, and auxin-repressed protein were identified. Two CAI genes showed little or no sequence homology to the previously sequenced plant genes. Transcripts of the CAI genes were strongly or preferentially induced in pepper tissues by infection with X. campestris pv. vesicatoria or Phytophthora capsici, and by abiotic elicitor treatment. In particular, most of the CAI genes were strongly induced in pepper tissues by ethephon and methyl jasmonate.
从感染了无毒黄单胞菌野油菜黄单胞菌辣椒叶的过敏反应（HR）损伤的辣椒cDNA文库中，分离出了在细菌感染的辣椒叶与健康叶中表现出差异表达的特定cDNA。在总共测试的282个cDNA克隆中，36个单个cDNA基因（13%）与来自细菌感染叶的cDNA探针强烈杂交或差异杂交。鉴定出了10个辣椒诱导（CAI）基因，它们编码假定的硫堇、脂质转移蛋白I和II、渗透素（PR-5）、I类几丁质酶、β-1,3-葡聚糖酶、SAR 8.2、杨梅素、富含亮氨酸重复序列蛋白和生长素抑制蛋白。两个CAI基因与先前测序的植物基因几乎没有序列同源性。CAI基因的转录本在辣椒组织中被野油菜黄单胞菌或辣椒疫霉感染以及通过非生物激发子处理后强烈或优先诱导。特别是，大多数CAI基因在辣椒组织中被乙烯利和茉莉酸甲酯强烈诱导。
