• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

黄瓜霜霉病抗性基因 O 与钙调蛋白相互作用并调控与植物免疫相关的细胞死亡。

Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity.

机构信息

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.

出版信息

Int J Mol Sci. 2019 Jun 19;20(12):2995. doi: 10.3390/ijms20122995.

DOI:10.3390/ijms20122995
PMID:31248151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6627319/
Abstract

Pathogen-induced cell death is closely related to plant disease susceptibility and resistance. The cucumber ( L.) mildew resistance locus O (CsMLO1) and calmodulin (CsCaM3) genes, as molecular components, are linked to nonhost resistance and hypersensitive cell death. In this study, we demonstrate that CsMLO1 interacts with CsCaM3 via yeast two-hybrid, firefly luciferase (LUC) complementation and bimolecular fluorescence complementation (BiFC) experiments. A subcellular localization analysis of green fluorescent protein (GFP) fusion reveals that is transferred from the cytoplasm to the plasma membrane in , and green fluorescence is significantly attenuated via the coexpression of and negatively regulates expression in transiently transformed cucumbers, and hypersensitive cell death is disrupted by and/or expression under infection. Additionally, silencing significantly enhances the expression of reactive oxygen species (ROS)-related genes (, , and ), defense marker genes ( and ) and callose deposition-related gene () in infected cucumbers. These results suggest that the interaction of CsMLO1 with CsCaM3 may act as a cell death regulator associated with plant immunity and disease.

摘要

病原诱导的细胞死亡与植物的感病性和抗病性密切相关。黄瓜（ L.）霜霉病抗性位点 O（CsMLO1）和钙调蛋白（CsCaM3）基因作为分子成分，与非寄主抗性和过敏细胞死亡有关。在这项研究中，我们通过酵母双杂交、萤火虫荧光素酶（LUC）互补和双分子荧光互补（BiFC）实验证明了 CsMLO1 与 CsCaM3 相互作用。绿色荧光蛋白（GFP）融合的亚细胞定位分析表明，在 中，从细胞质转移到质膜，并且通过共表达 ，绿色荧光明显减弱，负调控瞬时转化黄瓜中 的表达，并且在 感染下，通过 和/或 表达破坏过敏细胞死亡。此外，沉默显著增强了感染黄瓜中活性氧（ROS）相关基因（、、和）、防御标记基因（和）和胼胝质沉积相关基因（）的表达。这些结果表明，CsMLO1 与 CsCaM3 的相互作用可能作为与植物免疫和疾病相关的细胞死亡调节剂发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/adda25c9963b/ijms-20-02995-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/0729fb6fdc8c/ijms-20-02995-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/14aec0c920ec/ijms-20-02995-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/ea0e7ceead74/ijms-20-02995-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/0338122fad93/ijms-20-02995-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/3326dd5d76d3/ijms-20-02995-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/d27a34bf3101/ijms-20-02995-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/572af4d4e0dc/ijms-20-02995-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/5bceee75421d/ijms-20-02995-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/590ec78b658e/ijms-20-02995-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/67091cd972f4/ijms-20-02995-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/adda25c9963b/ijms-20-02995-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/0729fb6fdc8c/ijms-20-02995-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/14aec0c920ec/ijms-20-02995-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/ea0e7ceead74/ijms-20-02995-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/0338122fad93/ijms-20-02995-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/3326dd5d76d3/ijms-20-02995-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/d27a34bf3101/ijms-20-02995-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/572af4d4e0dc/ijms-20-02995-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/5bceee75421d/ijms-20-02995-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/590ec78b658e/ijms-20-02995-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/67091cd972f4/ijms-20-02995-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e883/6627319/adda25c9963b/ijms-20-02995-g011.jpg

相似文献

1
Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity.黄瓜霜霉病抗性基因 O 与钙调蛋白相互作用并调控与植物免疫相关的细胞死亡。
Int J Mol Sci. 2019 Jun 19;20(12):2995. doi: 10.3390/ijms20122995.
2
Mildew Resistance Locus O Genes and Are Negative Modulators of the Defense Response to .黑曲霉抗霉基因 O 和是对的防御反应的负调节剂。
Int J Mol Sci. 2019 Sep 26;20(19):4793. doi: 10.3390/ijms20194793.
3
Pepper mildew resistance locus O interacts with pepper calmodulin and suppresses Xanthomonas AvrBsT-triggered cell death and defense responses.胡椒褪绿斑驳病毒抗性位点 O 与胡椒钙调蛋白相互作用，抑制黄单胞菌 AvrBsT 触发的细胞死亡和防御反应。
Planta. 2014 Oct;240(4):827-39. doi: 10.1007/s00425-014-2134-y. Epub 2014 Jul 30.
4
Multiplex gene editing reveals cucumber MILDEW RESISTANCE LOCUS O family roles in powdery mildew resistance.多重基因编辑揭示黄瓜白粉病抗性基因 O 家族在白粉病抗性中的作用。
Plant Physiol. 2024 May 31;195(2):1069-1088. doi: 10.1093/plphys/kiae047.
5
Loss-of-Function Mutations in CsMLO1 Confer Durable Powdery Mildew Resistance in Cucumber (Cucumis sativus L.).黄瓜（Cucumis sativus L.）中CsMLO1功能缺失突变赋予对白粉病的持久抗性
Front Plant Sci. 2015 Dec 22;6:1155. doi: 10.3389/fpls.2015.01155. eCollection 2015.
6
Pepper heat shock protein 70a interacts with the type III effector AvrBsT and triggers plant cell death and immunity.辣椒热激蛋白70a与III型效应蛋白AvrBsT相互作用，引发植物细胞死亡和免疫反应。
Plant Physiol. 2015 Feb;167(2):307-22. doi: 10.1104/pp.114.253898. Epub 2014 Dec 9.
7
Analysis of Protein Synthesis in Cucumber Leaves after Inoculation with Corynespora cassiicola: A Proteomic Approach.接种古巴拟盘多毛孢后黄瓜叶片蛋白质合成分析：一种蛋白质组学方法。
Biochemistry (Mosc). 2019 Aug;84(8):963-977. doi: 10.1134/S0006297919080121.
8
Expression and functional analysis of the transcription factor-encoding Gene CsERF004 in cucumber during Pseudoperonospora cubensis and Corynespora cassiicola infection.黄瓜转录因子编码基因CsERF004在瓜类霜霉病菌和多主棒孢菌侵染过程中的表达及功能分析
BMC Plant Biol. 2017 Jun 5;17(1):96. doi: 10.1186/s12870-017-1049-8.
9
Nucleotide-Binding Leucine-Rich Repeat Genes and Are Positive Modulators in the Defense Response to .核苷酸结合富含亮氨酸重复序列基因是对 的防御反应的正调节剂。
Int J Mol Sci. 2021 Apr 13;22(8):3986. doi: 10.3390/ijms22083986.
10
CsPM5.2, a phosphate transporter protein-like gene, promotes powdery mildew resistance in cucumber.CsPM5.2，一种磷酸转运蛋白样基因，可促进黄瓜对白粉病的抗性。
Plant J. 2024 Mar;117(5):1487-1502. doi: 10.1111/tpj.16576. Epub 2023 Dec 4.

引用本文的文献

1
Enhancing powdery mildew resistance in soybean by targeted mutation of MLO genes using the CRISPR/Cas9 system.利用 CRISPR/Cas9 系统靶向突变 MLO 基因增强大豆对白粉病的抗性。
BMC Plant Biol. 2023 Nov 3;23(1):533. doi: 10.1186/s12870-023-04549-5.
2
Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-CALMODULIN2 interaction by various in vitro and in vivo protein-protein interaction assays.通过各种体外和体内蛋白质-蛋白质相互作用试验对拟南芥MLO2-钙调蛋白2相互作用进行全面比较评估。
Biochem J. 2023 Oct 31;480(20):1615-1638. doi: 10.1042/BCJ20230255.
3
Comprehensive comparative assessment of the MLO2-calmodulin interaction by various and protein-protein interaction assays.

本文引用的文献

1
Mildew Resistance Locus O Genes and Are Negative Modulators of the Defense Response to .黑曲霉抗霉基因 O 和是对的防御反应的负调节剂。
Int J Mol Sci. 2019 Sep 26;20(19):4793. doi: 10.3390/ijms20194793.
2
Analysis of Protein Synthesis in Cucumber Leaves after Inoculation with Corynespora cassiicola: A Proteomic Approach.接种古巴拟盘多毛孢后黄瓜叶片蛋白质合成分析：一种蛋白质组学方法。
Biochemistry (Mosc). 2019 Aug;84(8):963-977. doi: 10.1134/S0006297919080121.
3
The Two Translationally Controlled Tumor Protein Genes, and , Are Negative Modulators in the Defense Response to .
通过各种蛋白质-蛋白质相互作用分析对MLO2-钙调蛋白相互作用进行全面比较评估。
bioRxiv. 2023 Jan 25:2023.01.25.525488. doi: 10.1101/2023.01.25.525488.
4
SlMYC2 interacted with the promoter and mediated JA signaling to regulate growth and fruit quality in tomato.SlMYC2与启动子相互作用并介导茉莉酸信号传导，以调控番茄的生长和果实品质。
Front Plant Sci. 2022 Oct 27;13:1013445. doi: 10.3389/fpls.2022.1013445. eCollection 2022.
5
Engineering plant immune circuit: walking to the bright future with a novel toolbox.工程植物免疫回路：用新型工具箱走向光明未来。
Plant Biotechnol J. 2023 Jan;21(1):17-45. doi: 10.1111/pbi.13916. Epub 2022 Sep 27.
6
Rapid Investigation of Functional Roles of Genes in Regulation of Leaf Senescence Using Arabidopsis Protoplasts.利用拟南芥原生质体快速研究基因在叶片衰老调控中的功能作用
Front Plant Sci. 2022 Mar 17;13:818239. doi: 10.3389/fpls.2022.818239. eCollection 2022.
7
Mildew Resistance Locus O Genes and Are Negative Modulators of the Defense Response to .黑曲霉抗霉基因 O 和是对的防御反应的负调节剂。
Int J Mol Sci. 2019 Sep 26;20(19):4793. doi: 10.3390/ijms20194793.
8
Genome-Wide Identification and Characterization of Long Non-Coding RNAs in Peanut.花生中长链非编码 RNA 的全基因组鉴定和特征分析。
Genes (Basel). 2019 Jul 15;10(7):536. doi: 10.3390/genes10070536.
两个翻译调控肿瘤蛋白基因，即 和 ，是对 防御反应中的负调节因子。
Front Plant Sci. 2018 Apr 25;9:544. doi: 10.3389/fpls.2018.00544. eCollection 2018.
4
Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber.黄瓜对姜疫病菌响应的转录组和 miRNA 分析。
Sci Rep. 2018 May 17;8(1):7798. doi: 10.1038/s41598-018-26080-6.
5
Induction of Defense-Related Physiological and Antioxidant Enzyme Response against Powdery Mildew Disease in Okra ( L.) Plant by Using Chitosan and Potassium Salts.壳聚糖和钾盐诱导秋葵植株对白粉病产生防御相关生理和抗氧化酶反应
Mycobiology. 2017 Dec;45(4):409-420. doi: 10.5941/MYCO.2017.45.4.409. Epub 2017 Dec 31.
6
Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species.拟南芥 MLO2 是细胞外活性氧敏感性的负调控因子。
Plant Cell Environ. 2018 Apr;41(4):782-796. doi: 10.1111/pce.13144. Epub 2018 Feb 22.
7
Root respiratory burst oxidase homologue-dependent H2O2 production confers salt tolerance on a grafted cucumber by controlling Na+ exclusion and stomatal closure.根呼吸爆发氧化酶同源物依赖的 H2O2 产生通过控制 Na+ 排斥和气孔关闭赋予嫁接黄瓜耐盐性。
J Exp Bot. 2018 Jun 19;69(14):3465-3476. doi: 10.1093/jxb/erx386.
8
Salicylic acid seed priming instigates defense mechanism by inducing PR-Proteins in Solanum melongena L. upon infection with Verticillium dahliae Kleb.水杨酸种子引发通过在茄子感染大丽轮枝菌时诱导病程相关蛋白来激发防御机制。
Plant Physiol Biochem. 2017 Aug;117:12-23. doi: 10.1016/j.plaphy.2017.05.012. Epub 2017 May 24.
9
Aniline blue and Calcofluor white staining of callose and cellulose in the streptophyte green algae and .链形植物绿藻中胼胝质和纤维素的苯胺蓝及荧光增白剂染色 以及 。 （你提供的原文似乎不完整，翻译后的内容中会保留原文的不完整性表述。）
Bio Protoc. 2016 Oct 20;6(20). doi: 10.21769/BioProtoc.1969.
10
CsWRKY46, a WRKY transcription factor from cucumber, confers cold resistance in transgenic-plant by regulating a set of cold-stress responsive genes in an ABA-dependent manner.CsWRKY46是一种来自黄瓜的WRKY转录因子，它通过以脱落酸依赖的方式调控一组冷胁迫响应基因，赋予转基因植物抗寒能力。
Plant Physiol Biochem. 2016 Nov;108:478-487. doi: 10.1016/j.plaphy.2016.08.013. Epub 2016 Aug 27.