Kim Soo-Yong, Kim Young-Cheol, Lee Jeong-Hee, Oh Sang-Keun, Chung Eunsook, Lee Sanghyeob, Lee Yong-Hwan, Choi Doil, Park Jeong Mee
Plant Genomics Lab., Division of Genomics, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusung, Daejon 305-600, Republic of Korea.
Biochim Biophys Acta. 2005 Jul 10;1729(3):141-6. doi: 10.1016/j.bbaexp.2005.04.009.
We isolated a cDNA clone, CaRAV1, which exhibited significant similarity to those of Arabidopsis RAV proteins containing AP2/ERF and B3-like DNA-binding domains. CaRAV1 expression was rapidly and specifically induced in both host and non-host resistant responses against bacterial pathogens in the chili pepper plant. CaRAV1 also strongly increased following salicylic acid and ethephon treatments, whereas methyl-jasmonate only had mild effects. Furthermore, CaRAV1 transcript levels were also investigated in response to ABA and abiotic stress. No significant CaRAV1 expression was evident following ABA, mannitol, or cold treatments. These observations collectively provide initial evidence that the pepper RAV transcription factor homolog may function in plant defense responses.
我们分离出一个cDNA克隆CaRAV1，它与拟南芥中含有AP2/ERF和B3样DNA结合结构域的RAV蛋白具有显著相似性。在辣椒植株对细菌病原体的宿主和非宿主抗性反应中，CaRAV1的表达均被迅速且特异性地诱导。水杨酸和乙烯利处理后，CaRAV1也显著增加，而茉莉酸甲酯的影响较小。此外，还研究了CaRAV1转录水平对脱落酸（ABA）和非生物胁迫的响应。ABA、甘露醇或冷处理后，未观察到明显的CaRAV1表达。这些观察结果共同提供了初步证据，表明辣椒RAV转录因子同源物可能在植物防御反应中发挥作用。
