Sánchez F, López M J, del Campo J, Gutiérrez J L, Moreno M N, Vázquez R
Department of Human Anatomy and Histology, Faculty of Medicine, Campus Unamuno, Salamanca.
Neuropeptides. 1999 Dec;33(6):487-91. doi: 10.1054/npep.1999.0767.
Nitric oxide (NO) plays an important role in the maintenance of autonomic homeostasis of the body. NO-producing neurons are activated in the brain during exposure to different levels of environmentally produced stimulations. These facts prompted the authors to investigate the levels of activation of NO-producing neurons of the paraventricular nucleus of the hypothalamus (PVN) following change of environment and darkness. Cryostat sections were cut and processed for the histochemical detection of the NADPH-diaphorase (ND)-activity. Following change of the environment and darkness for 12 and 24 h, statistical analysis displayed a significant increase in the number of ND-neurons, especially in the posterior magnocellular and the lateral parvicellular subdivisions of the PVN. These data indicate that the ND-neurons of the PVN are influenced by changes of the environment and darkness.
一氧化氮（NO）在维持机体自主稳态中发挥着重要作用。在暴露于不同水平的环境刺激时，脑内产生NO的神经元会被激活。这些事实促使作者研究环境变化和黑暗后下丘脑室旁核（PVN）中产生NO的神经元的激活水平。制作冰冻切片并进行处理，以进行NADPH-黄递酶（ND）活性的组织化学检测。在环境变化和黑暗持续12小时和24小时后，统计分析显示ND神经元数量显著增加，尤其是在PVN的后部大细胞和外侧小细胞亚区。这些数据表明，PVN的ND神经元受环境变化和黑暗的影响。
