Sánchez F, Martinez M E, Rubio M, Carretero J, Moreno M N, Vázquez R
Department of Human Anatomy and Histology, Faculty of Medicine, Campus Unamuno, Salamanca, Spain.
Neurosci Lett. 1998 Sep 4;253(2):75-8. doi: 10.1016/s0304-3940(98)00581-3.
Based on previous studies demonstrating that reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase (ND) activity is modulated by estradiol and the discovery of a new subtype of estrogen receptor highly expressed in the paraventricular nucleus of the hypothalamus (PVN), a possible estrogen influence on this activity was investigated in the neuronal populations (magno- and parvicellular) of this nucleus. Cryostat sections were cut and processed for the histochemical detection of the ND activity. Following ovariectomy (14 days), numerical data displayed a slight decrease in the number of ND-neurons, especially in the posterior magnocellular and the medial parvicellular subdivisions, which was reversed after daily treatment with estradiol benzoate. Administration of estradiol benzoate to male rats (14 days) induced a significant increase (P < 0.05) in the number of ND-neurons, mainly at the level of the posterior magnocellular subdivision. These data indicate that paraventricular ND-neurons are influenced by estradiol.
基于先前的研究表明还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-黄递酶（ND）活性受雌二醇调节，以及发现一种在下丘脑室旁核（PVN）中高表达的新型雌激素受体，本研究探讨了雌激素对该核团神经元群体（大细胞和小细胞）中这种活性的可能影响。制作了恒冷箱切片并进行处理，以对ND活性进行组织化学检测。卵巢切除术后（14天），数值数据显示ND神经元数量略有减少，尤其是在后侧大细胞和内侧小细胞亚区，而在每日注射苯甲酸雌二醇后这种减少得以逆转。给雄性大鼠注射苯甲酸雌二醇（14天）导致ND神经元数量显著增加（P<0.05），主要是在后侧大细胞亚区水平。这些数据表明室旁核ND神经元受雌二醇影响。
