Michie A M, Carlyle J R, Schmitt T M, Ljutic B, Cho S K, Fong Q, Zúñiga-Pflücker J C
Department of Immunology, University of Toronto, Toronto, Ontario, Canada.
J Immunol. 2000 Feb 15;164(4):1730-3. doi: 10.4049/jimmunol.164.4.1730.
We recently described a population of fetal thymocytes with a CD117+NK1.1+CD90lowCD25- phenotype, which were shown to contain committed T cell and NK cell progenitors. However, the characterization of a single cell with a restricted T and NK cell precursor potential was lacking. Here, using an in vitro model for T and NK cell differentiation, we provide conclusive evidence demonstrating the existence of a clonal lineage-restricted T and NK cell progenitor. These results establish that fetal thymocytes with a CD117+NK1.1+CD90lowCD25- phenotype represent bipotent T and NK cell progenitors.
我们最近描述了一群具有CD117+NK1.1+CD90lowCD25-表型的胎儿胸腺细胞，已证明其含有定向T细胞和NK细胞祖细胞。然而，缺乏对具有受限T和NK细胞前体潜能的单个细胞的表征。在此，我们使用T细胞和NK细胞分化的体外模型，提供了确凿证据，证明存在克隆性谱系受限的T和NK细胞祖细胞。这些结果证实，具有CD117+NK1.1+CD90lowCD25-表型的胎儿胸腺细胞代表双能T和NK细胞祖细胞。
