Bianchini F, Elmståhl S, Martinez-Garciá C, van Kappel A L, Douki T, Cadet J, Ohshima H, Riboli E, Kaaks R
International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France.
Carcinogenesis. 2000 Feb;21(2):321-4. doi: 10.1093/carcin/21.2.321.
In order to investigate whether oxidative damage is associated with differences in antioxidant intake, we measured the levels of 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) in lymphocytes and alpha-tocopherol and several carotenoids in plasma of women with different dietary habits. We found that women from Granada (Spain), a region with a typically Mediterranean diet, had significantly higher levels of 8-oxodGuo compared with Malmö (Sweden), a region with a Northern European dietary intake pattern (2.30 +/- 0.78 versus 1.59 +/- 1.01 8-oxodGuo/10(-6) deoxyguanosine). Levels of plasma alpha-tocopherol and carotenoids were higher in Granada and these values were significantly positively correlated with levels of 8-oxodGuo. Our results do not support the hypothesis that a Mediterranean diet rich in alpha-tocopherol and carotenoids protects cells against oxidative DNA damage. It is possible, however, that consumption of foods other than fruits and vegetables, including fats, are responsible for the higher levels of 8-oxodGuo in Granada. Further studies are warranted to better elucidate the role of antioxidants in the modulation of oxidative stress in vivo.
为了研究氧化损伤是否与抗氧化剂摄入量的差异有关，我们测量了不同饮食习惯女性淋巴细胞中8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代脱氧鸟苷，8-oxodGuo）的水平以及血浆中α-生育酚和几种类胡萝卜素的水平。我们发现，来自西班牙格拉纳达（典型的地中海饮食地区）的女性，与北欧饮食摄入模式地区瑞典马尔默的女性相比，其8-oxodGuo水平显著更高（分别为2.30±0.78与1.59±1.01 8-oxodGuo/10⁻⁶脱氧鸟苷）。格拉纳达女性血浆中α-生育酚和类胡萝卜素水平更高，且这些值与8-oxodGuo水平显著正相关。我们的结果不支持富含α-生育酚和类胡萝卜素的地中海饮食可保护细胞免受氧化性DNA损伤这一假设。然而，有可能除水果和蔬菜之外的食物（包括脂肪）的摄入导致了格拉纳达女性8-oxodGuo水平较高。有必要进行进一步研究以更好地阐明抗氧化剂在体内调节氧化应激中的作用。
