Spragg R G, Smith R M, Harris K, Lewis J, Häfner D, Germann P
Department of Medicine, University of California San Diego School of Medicine and San Diego Veterans Affairs HealthCare System, San Diego, California 92161, USA.
J Appl Physiol (1985). 2000 Feb;88(2):674-81. doi: 10.1152/jappl.2000.88.2.674.
Synthetic surfactants allow examination of the effects of specific components of natural surfactant. To determine whether surfactant containing apoprotein C, dipalmitoyl-phosphatidylcholine, phosphatidylglycerol, and palmitic acid restores gas-exchanging function in acute lung injury (ALI), we administered such surfactant (in doses of 50 or 100 mg/kg and in volumes from 1 to 6 ml/kg) or phospholipid (PL) alone, by intratracheal instillation, to pigs with ALI induced by massive saline lavage. Animals ventilated with 100% O(2) and receiving 1, 2, 4, or 6 ml/kg of 50 mg/kg recombinant surfactant apoprotein C (rSP-C) surfactant or 2 ml/kg of 50 mg/kg PL (control) had mean arterial PO(2) values, 4 h after treatment, of 230, 332, 130, 142, or 86 Torr, respectively. Animals receiving 1, 2, or 4 ml/kg of 100 mg/kg rSP-C surfactant or 2 ml/kg of 100 mg/kg PL (control) had mean arterial PO(2) values of 197, 214, 148, or 88 Torr, respectively. Surfactant PL distribution was homogeneous. Hyaline membrane formation was reduced in treated animals. Thus, in this model of ALI, rSP-C with PL has the capacity to improve gas exchange and possibly modify lung injury.
合成表面活性剂有助于研究天然表面活性剂特定成分的作用。为了确定含有载脂蛋白C、二棕榈酰磷脂酰胆碱、磷脂酰甘油和棕榈酸的表面活性剂能否恢复急性肺损伤（ALI）中的气体交换功能，我们通过气管内滴注，将这种表面活性剂（剂量为50或100mg/kg，体积为1至6ml/kg）或单独的磷脂（PL）给予经大量盐水灌洗诱导ALI的猪。用100%氧气通气并接受1、2、4或6ml/kg的50mg/kg重组表面活性剂载脂蛋白C（rSP-C）表面活性剂或2ml/kg的50mg/kg PL（对照）的动物，治疗后4小时的平均动脉血氧分压值分别为230、332、130、142或86托。接受1、2或4ml/kg的100mg/kg rSP-C表面活性剂或2ml/kg的100mg/kg PL（对照）的动物，平均动脉血氧分压值分别为197、214、148或88托。表面活性剂PL分布均匀。治疗动物的透明膜形成减少...因此rSP-C与PL在ALI模型中具有改善气体交换并可能改变肺损伤的能力。 （原文最后一句“Thus, in this model of ALI, rSP-C with PL has the capacity to improve gas exchange and possibly modify lung injury.”中“possibly modify lung injury”后似乎缺少内容，但按照要求直接翻译）
