Halliwill J R
Department of Anesthesiology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
J Appl Physiol (1985). 2000 Feb;88(2):767-73. doi: 10.1152/jappl.2000.88.2.767.
The goal of this study was to merge the methods currently used to assess beat-by-beat changes in muscle sympathetic nerve activity with a signal-averaging approach and overcome the inherent subjectivity and time-consuming nature of manual analysis of baroreflex-mediated sympathetic responses in humans. This is a retrospective study using data obtained during two prior studies [J. R. Halliwill, J. A. Taylor, and D. L. Eckberg. J. Physiol. (Lond.) 495: 279-288, 1996; C. T. Minson, J. R. Halliwill, T. Young, and M. J. Joyner. FASEB J. 13: A1044, 1999]. Beat-by-beat arterial pressure (Finapres device) and muscle sympathetic nerve activity (microneurography) were recorded in seven healthy, nonsmoking, normotensive subjects (2 men, 5 women) between the ages of 23 and 32 yr during arterial pressure changes induced by bolus injections of nitroprusside and phenylephrine. The muscle sympathetic nerve activity-diastolic pressure relationship was analyzed by both the traditional manual detection method and a novel segregated signal-averaging method. The results show the two analysis approaches are highly correlated across subjects (r = 0.914, P < 0. 05) and are in close agreement [slope for manual detection -6.17 +/- 0.91 (SE) vs. slope for segregated signal averaging -5.98 +/- 0.83 total integrated activity. beat(-1). mmHg(-1); P = 0.60]. However, a considerable time savings is seen with the new method (min vs. h). Segregated signal averaging as developed here provides a valid alternative to "by-hand" analysis of beat-by-beat changes in muscle sympathetic nerve activity that occur during dynamic baroreflex-mediated changes in sympathetic outflow. This approach provides an objective, rapid method to analyze nerve recordings.
本研究的目的是将目前用于评估肌肉交感神经活动逐搏变化的方法与信号平均法相结合，克服人体压力反射介导的交感反应手动分析中固有的主观性和耗时性。这是一项回顾性研究，使用了之前两项研究[J.R. Halliwill、J.A. Taylor和D.L. Eckberg。《生理学杂志》（伦敦）495: 279 - 288，1996；C.T. Minson、J.R. Halliwill、T. Young和M.J. Joyner。《美国实验生物学会联合会杂志》13: A1044，1999]中获得的数据。在23至32岁的7名健康、不吸烟、血压正常的受试者（2名男性，5名女性）中，记录了在静脉注射硝普钠和去氧肾上腺素引起动脉压变化期间的逐搏动脉压（Finapres设备）和肌肉交感神经活动（微神经ography）。通过传统的手动检测方法和一种新的分离信号平均方法分析了肌肉交感神经活动与舒张压的关系。结果表明，两种分析方法在受试者之间高度相关（r = 0.914，P < 0.05），并且结果非常一致[手动检测的斜率为-6.17±0.91（标准误），而分离信号平均的斜率为-5.98±0.83总积分活动。次-1。毫米汞柱-1；P = 0.60]。然而，新方法节省了大量时间（分钟与小时）。本文开发的分离信号平均法为动态压力反射介导的交感神经流出变化期间肌肉交感神经活动逐搏变化的“手动”分析提供了一种有效的替代方法。这种方法提供了一种客观、快速的神经记录分析方法。
