Georgopoulos A
Zentralbl Bakteriol Orig A. 1978;242(3):387-93.
A micro agar diffusion method to determine antibiotic levels in only 0.02 ml of serum is described. With this technique, standard curves for Ampicillin, Penicillin G, Gentamicin, Tetracycline HCl and Amphotericin B were calculated. The method displays a good reproducibility with an error of less than 10%. To compare this technique with a conventional macro agar diffusion method fixed antibiotic concentrations in rabbit serum were examined. Corresponding results were obtained indicating the equivalence of this method, which thus proved to be suitable to determine antibiotic levels in small volumes of body fluids.
本文描述了一种仅用0.02毫升血清就能测定抗生素水平的微量琼脂扩散法。利用该技术，计算了氨苄青霉素、青霉素G、庆大霉素、盐酸四环素和两性霉素B的标准曲线。该方法具有良好的重现性，误差小于10%。为了将该技术与传统的常量琼脂扩散法进行比较，检测了兔血清中固定的抗生素浓度。获得了相应的结果，表明该方法具有等效性，因此被证明适用于测定少量体液中的抗生素水平。
