Zhao J, Carr P W
Department of Chemistry, University of Minnesota, Minneapolis 55414, USA.
Anal Chem. 2000 Jan 15;72(2):302-9. doi: 10.1021/ac9906734.
The chromatographic selectivities of polystyrene-coated zirconia (PS-ZrO2) have been investigated in detail by means of homologue series retention studies and studies of a variety of nonpolar aromatic positional isomers. This material has been compared to polybutadiene-coated zirconia, octadecyl- and phenyl-bonded silica, and polymer-based reversed phases. In addition, the separations of a wide variety of compound classes on PS-ZrO2 were compared with those on other aromatic and aliphatic RPLC phases. The PS-ZrO2 material exhibits substantial differences in selectivity toward nonpolar and polar analytes as compared to other phases, especially with respect to polybutadiene-coated zirconia (PBD-ZrO2). On PS-ZrO2, polynuclear aromatic hydrocarbons (PAHs) are retained much more than are alkylbenzenes, whereas on PBD-ZrO2 the opposite is observed. In addition, PS-ZrO2 has much greater selectivity than does PBD-ZrO2 for the separation of structural isomers which differ in the position of phenyl groups. In general, even though the carbon content of PS-ZrO2 is much lower than that of conventional reversed-phase materials, such as octadecyl-bonded silica (C18-SiO2), polar analytes exhibit sufficient retention and differential selectivity on PS-ZrO2 that resolution comparable to that on C18-SiO2 can be obtained but with substantially improved analysis times.
通过同系物系列保留研究以及对多种非极性芳香位置异构体的研究，对聚苯乙烯包覆氧化锆（PS-ZrO₂）的色谱选择性进行了详细研究。已将该材料与聚丁二烯包覆氧化锆、十八烷基键合硅胶和苯基键合硅胶以及聚合物基反相材料进行了比较。此外，还将PS-ZrO₂上多种化合物类别的分离与其他芳香族和脂肪族反相液相色谱（RPLC）相上的分离进行了比较。与其他相相比，PS-ZrO₂材料对非极性和极性分析物的选择性存在显著差异，尤其是与聚丁二烯包覆氧化锆（PBD-ZrO₂）相比。在PS-ZrO₂上，多核芳香烃（PAHs）的保留程度远高于烷基苯，而在PBD-ZrO₂上则观察到相反的情况。此外，对于在苯基位置上不同的结构异构体，PS-ZrO₂比PBD-ZrO₂具有更高的选择性。一般来说，尽管PS-ZrO₂的碳含量远低于传统反相材料，如十八烷基键合硅胶（C18-SiO₂），但极性分析物在PS-ZrO₂上仍表现出足够的保留和差异选择性，能够获得与C18-SiO₂相当的分离度，同时分析时间大幅缩短。
