Jackson Paul T, Carr Peter W
Chemistry Department, St. Olaf College, Northfield, MN 55057, USA.
J Chromatogr A. 2002 Jun 7;958(1-2):121-9. doi: 10.1016/s0021-9673(02)00392-8.
Retention factors of polar and non-polar mono- and di-substituted benzene derivatives were measured on carbon coated zirconia (C/ZrO2) and an alkyl modified silica using water-acetonitrile mobile phases. Published data on porous graphitic carbon phases (PGC) were used to facilitate comparisons between the two types of carbon media. This work showed that retention on both C/ZrO2 and PGC is much more sensitive to the solute polarizability, dipolarity, and shape than on aliphatic phases. For simple disubstituted benzenes there was no general clear cut advantage in terms of chromatographic selectivity to using a carbon-based phase over a bonded phase silica; however, the selectivities towards such isomers are quite different on the two types of media. In contradistinction to their effect on alkyl bonded phase retention, addition of a dipolar substituent and weak hydrogen bond acceptor to a benzene ring almost always increases the solute's retention on C/ZrO2 and PGC.
使用水 - 乙腈流动相，在碳包覆氧化锆（C/ZrO₂）和烷基改性硅胶上测量了极性和非极性单取代及二取代苯衍生物的保留因子。利用已发表的关于多孔石墨碳相（PGC）的数据来促进两种碳介质之间的比较。这项工作表明，与在脂肪族相上相比，C/ZrO₂和PGC上的保留对溶质的极化率、偶极矩和形状更为敏感。对于简单的二取代苯，就色谱选择性而言，使用碳基相相对于键合相硅胶并没有普遍明显的优势；然而，在这两种介质上对这类异构体的选择性却大不相同。与它们对烷基键合相保留的影响相反，向苯环中添加一个偶极取代基和弱氢键受体几乎总是会增加溶质在C/ZrO₂和PGC上的保留。
