Paritpokee N, Tangkijvanich P, Teerasaksilp S, Wiwanitkit V, Lertmaharit S, Tosukhowong P
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
J Med Assoc Thai. 1999 Dec;82(12):1241-6.
Fast liver alkaline phosphatase isoenzyme was measured by cellulose acetate electrophoresis in the sera obtained from 84 patients with specific hepatobiliary diseases and 10 control subjects. The mean value of this isoenzyme in patients with malignant extrahepatic obstruction was 130.58 +/- 107.08 U/L, significantly higher than that of patients with benign extrahepatic obstruction (65.63 +/- 34.14 U/L), as well as patients with intrahepatic cholestasis and infiltrative liver cancers (65.31 +/- 38.11 U/L and 48.47 +/- 36.85 U/L, respectively). Furthermore, we could not detect this isoenzyme in normal individuals. When 100 U/L was used as a cut-off value to discriminate between patients with malignant extrahepatic obstruction and the remaining hepatobiliary disorders, the sensitivity, specificity and accuracy of the test were 63 per cent, 84 per cent and 80 per cent, respectively. It is concluded that the fast liver isoenzyme could be a useful marker in diagnosis of malignant extrahepatic obstruction.
采用醋酸纤维素电泳法检测了84例特定肝胆疾病患者及10例对照者血清中的快速肝碱性磷酸酶同工酶。肝外恶性梗阻患者该同工酶的平均值为130.58±107.08 U/L，显著高于肝外良性梗阻患者（65.63±34.14 U/L）以及肝内胆汁淤积和浸润性肝癌患者（分别为65.31±38.11 U/L和48.47±36.85 U/L）。此外，正常个体中未检测到该同工酶。以100 U/L作为区分肝外恶性梗阻患者与其余肝胆疾病的临界值时，该检测的灵敏度、特异度和准确度分别为63%、84%和80%。结论是快速肝同工酶可能是诊断肝外恶性梗阻的有用标志物。
