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[通过免疫荧光法测定细胞内免疫球蛋白。1. 针对副蛋白血症性成血细胞增多症患者骨髓制剂中人类G、A和M类免疫球蛋白的单特异性发光抗血清的标准化]

[Determinaton of intracellular immunoglobulins by means of immunofluorescence. 1. The standardization of monospecific luminescent antisera against human G-, A- and M-class immunoglobulins in bone marrow preparations from patients with paraproteinemic hemoblastosis].

作者信息

Liubetskiĭ V B, Zhuravlev V S, Novikova A V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978(12):121-6.

PMID:106607
Abstract

To assess the specificity and the activity of fluorescent antisera against human G-, A-, M-classes the authors used in the capacity of a substrate, containing specific antigen preparations, bone marrow cells of patients with paraproteinemic hemoblastosis. The method of making the preparations used permitted sufficiently objective assessment of the activity and specificity of the mentioned monospecific fluorescent conjugates. As revealed, the bone marrow preparations could be stored at -70 degrees C for over one year.

摘要

为评估荧光抗血清对人G、A、M类的特异性和活性,作者使用含特定抗原制剂的副蛋白血症成血细胞增多症患者骨髓细胞作为底物。所采用的制备制剂的方法能够充分客观地评估上述单特异性荧光结合物的活性和特异性。结果表明,骨髓制剂可在-70℃下保存一年以上。

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An immunofluorescence study on intracellular immunoglobulins in human bone marrow cells.
Ann N Y Acad Sci. 1971 Jun 21;177:290-305. doi: 10.1111/j.1749-6632.1971.tb35059.x.

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