Kotz C M, Wang C F, Briggs J E, Levine A S, Billington C J
Minnesota Obesity Center, Veterans Affairs Medical Center, Minneapolis 55417, USA.
Am J Physiol Regul Integr Comp Physiol. 2000 Feb;278(2):R494-8. doi: 10.1152/ajpregu.2000.278.2.R494.
Neuropeptide Y (NPY) injected into the hypothalamic paraventricular nucleus (PVN) stimulates feeding and decreases uncoupling protein (UCP)-1 mRNA in brown adipose tissue (BAT). The present studies were undertaken to determine whether UCP-2 in white adipose tissue (WAT) and UCP-3 in muscle are regulated by NPY in the PVN. PVN-cannulated male Sprague-Dawley rats were injected with either saline or NPY (PVN, 117 pmol, 0.5 microl) every 6 h for 24 h. NPY in the PVN stimulated feeding and decreased UCP-1 mRNA in BAT independent of NPY-induced feeding. UCP-2 mRNA in WAT was unchanged by NPY. In acromiotrapezius muscle, NPY decreased UCP-3 mRNA, but this was reversed by restricting food intake to control levels. In biceps femoris muscle, NPY alone had no effect on UCP-3 mRNA, but UCP-3 mRNA was significantly increased in the NPY-treated rats that were restricted to control levels of intake. These results suggest that UCP-2 in WAT and UCP-3 in muscle are not subject to specific regulation by NPY in the PVN.
向下丘脑室旁核（PVN）注射神经肽Y（NPY）会刺激进食，并降低棕色脂肪组织（BAT）中解偶联蛋白（UCP）-1的信使核糖核酸（mRNA）水平。本研究旨在确定白色脂肪组织（WAT）中的UCP-2和肌肉中的UCP-3是否受PVN中NPY的调节。对通过套管连接PVN的雄性斯普拉格-道利大鼠每6小时注射一次生理盐水或NPY（PVN，117皮摩尔，0.5微升），持续24小时。PVN中的NPY刺激进食，并降低BAT中UCP-1的mRNA水平，且与NPY诱导的进食无关。WAT中的UCP-2 mRNA水平不受NPY影响。在肩峰斜方肌中，NPY降低了UCP-3 mRNA水平，但通过将食物摄入量限制在对照水平可使其逆转。在股二头肌中，单独使用NPY对UCP-3 mRNA没有影响，但在食物摄入量被限制在对照水平的NPY处理大鼠中，UCP-3 mRNA显著增加。这些结果表明，WAT中的UCP-2和肌肉中的UCP-3不受PVN中NPY的特异性调节。
