Han Z J, Rhee S, Liu K, Miles H T, Davies D R
Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):104-5. doi: 10.1107/s0907444999012895.
Single crystals of d(CTCCT(S)CCGCGCG).d(CGCGCGGAG) have been grown by the vapor-diffusion method using 2-methyl-2,4-pentanediol as a precipitant. The crystals are tetragonal, space group P4(2), with unit-cell parameters a = b = 53.8, c = 43.1 A, and diffract to 1.8 A resolution at a synchrotron X-ray beamline. In the crystal, the asymmetric unit contains one copy of the construct. The two halves of the structure are related by non-crystallographic twofold symmetry. These observations are consistent with the conclusion that the sequences of the 12-mer and 9-mer oligonucleotides form a duplex DNA at one end and a triplex DNA at the other end.
已使用2-甲基-2,4-戊二醇作为沉淀剂，通过气相扩散法生长出d(CTCCT(S)CCGCGCG).d(CGCGCGGAG)的单晶。晶体为四方晶系，空间群P4(2)，晶胞参数a = b = 53.8，c = 43.1 Å，在同步加速器X射线束线处衍射至1.8 Å分辨率。在晶体中，不对称单元包含该构建体的一个拷贝。结构的两半通过非晶体学二重对称性相关联。这些观察结果与以下结论一致：12聚体和9聚体寡核苷酸序列一端形成双链DNA，另一端形成三链DNA。
