Ito E, Kondo F, Harada K
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.
Toxicon. 2000 Jan;38(1):37-48. doi: 10.1016/s0041-0101(99)00084-7.
The purpose of this study was to investigate the distribution of microcystin-LR (MCLR) orally administered to mice using an immunostaining method. MCLR was orally dosed at 500 microg/kg to aged Balb/C and ICR mice and their lethality was 23.9%. The former was more sensitive to MCLR than the latter, suggesting that oral toxicity by MCLR is related to the animal strains tested, although the pathological and immunostaining changes were essentially the same in both strains. According to this method the distribution of MCLR and related compounds were indicated as the red staining. Particularly, livers of dead aged mice were intensively stained. The main route of absorption was considered to be the small intestine because the villi contained a large amount of MCLR in both surface epithelial cells and lamina propria, resulting in erosion. The absorbed MCLR was contained in blood plasma and moved to the liver, lung, and heart, and finally to capillaries of the whole body. Excretion of MCLR was shown in the mucous from goblet cells in both the small intestine and large intestine.
本研究的目的是使用免疫染色方法研究口服给予小鼠的微囊藻毒素-LR（MCLR）的分布情况。将MCLR以500微克/千克的剂量口服给予老年Balb/C和ICR小鼠，其致死率为23.9%。前者对MCLR比后者更敏感，这表明MCLR的口服毒性与所测试的动物品系有关，尽管两种品系的病理和免疫染色变化基本相同。根据这种方法，MCLR及相关化合物的分布表现为红色染色。特别是，死亡老年小鼠的肝脏染色强烈。吸收的主要途径被认为是小肠，因为小肠绒毛的表面上皮细胞和固有层中都含有大量的MCLR，导致肠绒毛糜烂。吸收的MCLR存在于血浆中，并转移至肝脏、肺和心脏，最终到达全身毛细血管。MCLR通过小肠和大肠杯状细胞的黏液排出。
