Becquart P, Hocini H, Lévy M, Sépou A, Kazatchkine M D, Bélec L
Institut National de la Santé et de la Recherche Médicale U430, Hôpital Broussais, Paris, France.
J Infect Dis. 2000 Feb;181(2):532-9. doi: 10.1086/315255.
The immune response to human immunodeficiency virus (HIV) type 1 was evaluated in breast milk from HIV-infected African mothers who had transmitted and those who had not transmitted HIV to their children through breast-feeding. The levels, specific activities against gp160 and 2 HIV-derived peptides from gp41 and gp120 (V3 loop), and inhibitory activity toward viral transcytosis in vitro of secretory IgA (S-IgA) and IgG purified from breast milk were investigated in 8 transmitting mothers and 18 nontransmitting mothers. S-IgA and IgG antibodies to gp160 and to peptides were found in all breast milk samples. The specific activities of S-IgA and IgG to gp160 and peptides were similar between transmitting and nontransmitting mothers. No difference of the capacity of S-IgA and IgG to block HIV transcytosis in vitro was found between the 2 groups. These results suggest that humoral mucosal immunity to HIV does not appear as a predominant factor for protection against viral transmission through breast milk.
对通过母乳喂养将人类免疫缺陷病毒1型（HIV-1）传播给孩子的非洲感染HIV母亲以及未传播该病毒的母亲的母乳中的免疫反应进行了评估。对8名传播病毒的母亲和18名未传播病毒的母亲的母乳中分泌型IgA（S-IgA）和IgG的水平、针对gp160以及来自gp41和gp120（V3环）的2种HIV衍生肽的比活性，以及体外对病毒转胞吞作用的抑制活性进行了研究。在所有母乳样本中均发现了针对gp160和肽的S-IgA和IgG抗体。传播病毒的母亲和未传播病毒的母亲中，S-IgA和IgG对gp160和肽的比活性相似。两组之间未发现S-IgA和IgG体外阻断HIV转胞吞作用能力的差异。这些结果表明，针对HIV的体液黏膜免疫似乎不是防止通过母乳传播病毒的主要保护因素。
