Burrer Renaud, Haessig-Einius Sandrine, Aubertin Anne-Marie, Moog Christiane
EA3770, Institut de Virologie, Université Louis Pasteur, 67000 Strasbourg, France.
Virology. 2005 Mar 1;333(1):102-13. doi: 10.1016/j.virol.2004.12.034.
We analyzed the factors influencing the binding of polyclonal immunoglobulin (Ig)G from HIV-infected patients to primary isolates (PI) in capture assays and a potential correlation between this binding and neutralization. The fixation of antibodies (Abs) to viral particles was measured by quantifying the capture of 4 PI by purified IgG immobilized onto a plate or by analyzing the capture of IgG-virus complexes formed in solution. We found that the capture of virus and the formation of immune complexes is mainly achieved by Abs directed against the principal immunodominant domain (PID) of gp41. We have further compared the binding measured by these two methods and the neutralizing activity of our polyclonal IgG and found no correlation. Thus, capture assays, including the immune complex capture assay that is more representative of "physiological" conditions, cannot be used as surrogate method for the investigation of the neutralizing activity of Abs.
我们分析了在捕获试验中影响来自HIV感染患者的多克隆免疫球蛋白（Ig）G与原始分离株（PI）结合的因素，以及这种结合与中和作用之间的潜在相关性。通过定量固定在平板上的纯化IgG对4种PI的捕获量，或分析溶液中形成的IgG-病毒复合物的捕获情况，来测定抗体（Ab）与病毒颗粒的结合。我们发现，病毒的捕获和免疫复合物的形成主要是由针对gp41主要免疫显性结构域（PID）的抗体实现的。我们进一步比较了这两种方法测得的结合情况以及我们多克隆IgG的中和活性，未发现相关性。因此，捕获试验，包括更能代表“生理”条件的免疫复合物捕获试验，不能用作研究抗体中和活性的替代方法。
