Wagner D, Sprenger E, Merkle D
Acta Cytol. 1976 Jul-Aug;20(4):366-71.
Papanicolaou stained smears of various cervical lesions diagnosed as "suspicious" by routine cytology were reviewed with regard to different cell types leading to this diagnosis. The smears were then submitted to Feulgen hydrolysis and redyed by Acriflavin-SO2 for fluorescence-cytophotometry. In nine of 14 cases measured, the DNA content of all types of "suspicious" cells was increased with DNA modes at euploid levels of 2 n, 4 n and 8 n indicating that the "suspicious" cells in those cases are polyploid. However, in five cases aneuploid DNA-distribution patterns were found similar to those observed in carcinoma in situ or severe dysplasia. Since polyploidization may be considered as a cellular response to higher functional requirement (i.e. inflammation or regeneration) a "suspicious" cervical smear with a polyploid DNA-distribution pattern may reverse to normal cervical epithelium after normal conditions are restored. However, a "suspicious" smear with an aneuploid DNA-distribution pattern should be considered more seriously as being related to a precancerous lesion requiring immediate surgical treatment.
对经常规细胞学诊断为“可疑”的各种宫颈病变的巴氏染色涂片，就导致该诊断的不同细胞类型进行了复查。然后将涂片进行福尔根水解，并用吖啶黄 - 二氧化硫复染以进行荧光细胞光度测定。在测量的14例病例中的9例中，所有类型“可疑”细胞的DNA含量增加，DNA模式处于2n、4n和8n的整倍体水平，表明这些病例中的“可疑”细胞是多倍体。然而，在5例中发现了非整倍体DNA分布模式，类似于原位癌或重度发育异常中观察到的模式。由于多倍体化可被视为细胞对更高功能需求（即炎症或再生）的反应，具有多倍体DNA分布模式的“可疑”宫颈涂片在恢复正常情况后可能会恢复为正常宫颈上皮。然而，具有非整倍体DNA分布模式的“可疑”涂片应被更严肃地视为与需要立即手术治疗的癌前病变有关。
