水生腐殖酸和富里酸的在线尺寸排阻色谱/电喷雾电离质谱分析
On-line size-exclusion chromatography/electrospray ionisation mass spectrometry of aquatic humic and fulvic acids.
作者信息
Persson L, Alsberg T, Kiss G, Odham G
机构信息
Laboratory for Analytical Environmental Chemistry, Institute of Applied Environmental Research, Stockholm University, SE-106 91 Stockholm, Sweden.
出版信息
Rapid Commun Mass Spectrom. 2000;14(4):286-92. doi: 10.1002/(SICI)1097-0231(20000229)14:4<286::AID-RCM879>3.0.CO;2-C.
Isolated aquatic humic and fulvic acids were analysed with on-line size exclusion chromatography/electrospray ionisation mass spectrometry (SEC/ESI-MS). An eluent composition which enabled electrospray ionisation was identified. The SEC separation improved interpretability of mass spectra and may open up new possibilities for molecular weight determination of humic substances. A linear dose-response relationship over a factor of 20 was obtained and the limit of detection was 50ng/uL for humic and fulvic acids. Spectral changes due to different ionisation conditions (pH and cone voltage) were investigated. A natural water sample from a Swedish lake was analysed. Copyright 2000 John Wiley & Sons, Ltd.
采用在线尺寸排阻色谱/电喷雾电离质谱法（SEC/ESI-MS）对分离出的水生腐殖酸和富里酸进行了分析。确定了一种能够实现电喷雾电离的洗脱液组成。尺寸排阻色谱分离提高了质谱的可解释性，并可能为腐殖物质分子量的测定开辟新的可能性。获得了20倍因子范围内的线性剂量-反应关系，腐殖酸和富里酸的检测限为50ng/μL。研究了不同电离条件（pH值和锥电压）引起的光谱变化。对取自瑞典一个湖泊的天然水样进行了分析。版权所有2000约翰·威利父子有限公司。
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