Lou X
Laboratory of Macromolecular and Organic Chemistry and Dutch Polymer Institute, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
J Mass Spectrom. 2000 Nov;35(11):1308-12. doi: 10.1002/1096-9888(200011)35:11<1308::AID-JMS64>3.0.CO;2-W.
A direct sample fraction deposition method was developed for off-line size-exclusion chromatography (SEC)/matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. By using electrospray, the SEC eluent, together with a suitable matrix solution added coaxially, was directly deposited on the MALDI plate. Owing to the formation of very small droplets in electrospray, solvent evaporation is much faster. The fractionation volume in narrow-bore SEC, which can directly be collected in one MALDI spot, can easily be optimized in the range of a few microlitres. In addition, fairly homogeneous sample spots were obtained. The possible influence of composition variation of the SEC effluent on the analytical results using direct fraction deposition was investigated; no substantial effects were observed. The applicability of the method was demonstrated by characterizing a broad poly(methyl methacrylate) sample. Copyright 2000 John Wiley & Sons, Ltd.
开发了一种用于离线尺寸排阻色谱（SEC）/基质辅助激光解吸/电离（MALDI）飞行时间质谱的直接样品馏分沉积方法。通过电喷雾，将SEC洗脱液与同轴添加的合适基质溶液一起直接沉积在MALDI板上。由于电喷雾中形成非常小的液滴，溶剂蒸发要快得多。窄孔SEC中的分馏体积可以直接收集在一个MALDI点中，很容易在几微升范围内进行优化。此外，获得了相当均匀的样品点。研究了SEC流出物组成变化对使用直接馏分沉积的分析结果的可能影响；未观察到实质性影响。通过表征一种广泛的聚甲基丙烯酸甲酯样品证明了该方法的适用性。版权所有2000 John Wiley & Sons, Ltd.
