Schwietz L A, Goetz D W, Whisman B A, Reid M J
Allergy-Immunology Department, Wilford Hall Medical Center, Lackland AFB, Texas, USA.
Ann Allergy Asthma Immunol. 2000 Jan;84(1):87-93. doi: 10.1016/S1081-1206(10)62746-9.
There are increasing reports of Cupressaceae pollinosis from various geographic areas. Cross-reactivity among a limited number of species within the Cupressaceae family has been suggested. Juniperus ashei (mountain cedar) is the leading cause of respiratory allergy in South Texas.
This study examines in vivo and in vitro cross-reactivity among 12 Cupressaceae species, one Taxodiaceae species, one Pinaceae species, and an angiosperm.
Cross-reactivity among pollen extracts of mountain cedar (MC) and the other 14 trees was investigated by: (1) prick skin testing of each tree pollen extract in ten patients with MC pollinosis. (2) Ouchterlony gel immunodiffusion employing rabbit antisera to MC. (3) IgE immunoblotting using high-titer MC pooled human sera, and immunoblot inhibition after pre-incubation with MC protein. (4) Monoclonal antibody immunoblotting using a murine monoclonal antibody with strong affinity for the gp40 major allergen of MC.
Positive skin wheal-and-flare responses occurred to all 12 Cupressaceae and Japanese cedar (the Taxodiaceae), but not to the Pinaceae or the angiosperm. In Ouchterlony gels, lines of identity or partial identity formed between MC and all pollens except the Pinaceae and the angiosperm. Immunoblots demonstrated IgE binding to the 40 to 42 kD protein in each Cupressaceae, and to a parallel band in Japanese cedar at 43 to 46 kD. Immunoblot inhibition by MC pollen was complete for all trees. The monoclonal bound both the 40 to 42 kD protein in 11 of 12 Cupressaceae species and the 46 kD band in Japanese cedar, but bound no protein bands in the Pinaceae or the angiosperm.
Pollen proteins of the 12 Cupressaceae (including MC) and the Taxodiaceae (Japanese cedar) are extensively cross-reactive. In particular, the MC major allergen, gp40, is cross-reactive with 40 to 42 kD proteins of the other Cupressaceae and with the Japanese cedar major allergen of 46 kD. Component-based immunotherapy may someday allow a standard treatment for both Juniper-allergic and C. japonica-allergic patients.
来自不同地理区域的柏科花粉症报告日益增多。有研究表明柏科家族中有限数量的物种之间存在交叉反应性。杜松（山地雪松）是德克萨斯州南部呼吸道过敏的主要原因。
本研究检测了12种柏科植物、1种杉科植物、1种松科植物和1种被子植物之间的体内和体外交叉反应性。
通过以下方法研究了山地雪松（MC）与其他14种树木花粉提取物之间的交叉反应性：（1）对10名患有MC花粉症的患者进行每种树木花粉提取物的点刺皮肤试验。（2）使用兔抗MC血清进行欧氏凝胶免疫扩散试验。（3）使用高滴度MC混合人血清进行IgE免疫印迹，并在与MC蛋白预孵育后进行免疫印迹抑制试验。（4）使用对MC的gp40主要变应原具有强亲和力的鼠单克隆抗体进行单克隆抗体免疫印迹。
所有12种柏科植物和日本柳杉（杉科）均出现阳性皮肤风团和红晕反应，但松科植物和被子植物未出现。在欧氏凝胶中，MC与除松科植物和被子植物外的所有花粉之间形成了相同或部分相同的条带。免疫印迹显示IgE与每种柏科植物中的40至42kD蛋白以及日本柳杉中43至46kD的平行条带结合。MC花粉对所有树木的免疫印迹抑制均完全。单克隆抗体与12种柏科植物中的11种的40至42kD蛋白以及日本柳杉中的46kD条带结合，但未与松科植物或被子植物中的蛋白条带结合。
12种柏科植物（包括MC）和杉科植物（日本柳杉）的花粉蛋白具有广泛的交叉反应性。特别是，MC主要变应原gp40与其他柏科植物的40至42kD蛋白以及日本柳杉46kD的主要变应原具有交叉反应性。基于组分的免疫疗法可能有一天会为杜松过敏和日本柳杉过敏患者提供标准治疗。
