Goetz D W, Goetz M A, Whisman B A
Allergy-Immunology Department, Wilford Hall Medical Center, Lackland AFB, Texas, USA.
Ann Allergy Asthma Immunol. 1995 Sep;75(3):256-60.
Mountain cedar (Juniperus ashei) pollen is the principal aeroallergen in south central Texas from late December through February. The major mountain cedar allergen is a 40-kD glycoprotein, gp40.
To identify allergens in mountain cedar wood, leaves, and berries and to detect mountain cedar allergen in smoke from burning male or female trees.
SDS-PAGE plus mountain cedar human sIgE and monoclonal antibody immunoblots identified mountain cedar allergens within pollen and nonpollen tree part extracts.
IgE immunoblots identified a single wood allergen at 36 kD and three berry allergens at 36, 26-27, and 21 kD, in addition to known pollen allergens. Mountain cedar monoclonal antibody bound an allergen epitope present not only on 40, 33, and 28-kD pollen allergens, but also on 36 and 32-kD wood allergens, and the 26-27-kD berry allergen. Immunoblot studies detected no mountain cedar allergen in leaves and no allergen in smoke from burning male and female trees. Allergens constituted a much smaller percentage of extractable protein in wood and berries than in pollen.
Mountain cedar berry allergen content is too small to give credence to the ingestion of berries as a folk medicine treatment of mountain cedar pollinosis. In addition, while smoke from burning mountain cedar trees may be irritating, it contains no allergens that could cause allergic rhinoconjunctivitis.
从12月下旬到2月，山地雪松（杜松）花粉是德克萨斯州中南部主要的空气变应原。主要的山地雪松变应原是一种40-kD糖蛋白，即gp40。
鉴定山地雪松木材、树叶和浆果中的变应原，并检测燃烧雄树或雌树产生的烟雾中的山地雪松变应原。
SDS-PAGE结合山地雪松人sIgE和单克隆抗体免疫印迹法鉴定花粉和非花粉树部分提取物中的山地雪松变应原。
IgE免疫印迹法除了鉴定出已知的花粉变应原外，还鉴定出一种36 kD的单一木材变应原和三种36、26 - 27和21 kD的浆果变应原。山地雪松单克隆抗体结合的变应原表位不仅存在于40、33和28-kD的花粉变应原上，也存在于36和32-kD的木材变应原以及26 - 27-kD的浆果变应原上。免疫印迹研究未在树叶中检测到山地雪松变应原，也未在燃烧雄树和雌树产生的烟雾中检测到变应原。与花粉相比，变应原在木材和浆果中可提取蛋白质中所占的比例要小得多。
山地雪松浆果中的变应原含量太少，不足以支持将食用浆果作为治疗山地雪松花粉症的民间疗法。此外，虽然燃烧山地雪松树木产生的烟雾可能有刺激性，但其中不含有可导致变应性鼻结膜炎的变应原。
