Matsumoto A
Department of Anatomy, Juntendo University School of Medicine, Hongo, Tokyo, Japan.
Neurosci Lett. 2000 Jan 28;279(2):117-20. doi: 10.1016/s0304-3940(99)00965-9.
Phosphorylated cAMP response element-binding protein (CREB) immunoreactivity was examined in motoneurons of the spinal nucleus of the bulbocavernosus (SNB) in young and old male rats by immunohistochemistry. In young animals, intense CREB immunoreactivity was confined to the cell nucleus, but not in the nucleolus of SNB motoneurons. In old animals, both the intensity of CREB immunoreactivity in the nuclei and number of CREB immunoreactive nuclei of the SNB motoneurons were significantly reduced. A marked decline in expression of CREB in the aged SNB motoneurons suggests alternation of cAMP-mediated regulation of gene expression in the SNB system with advancing age.
通过免疫组织化学方法，检测了年轻和老年雄性大鼠球海绵体肌脊髓核（SNB）运动神经元中磷酸化环磷酸腺苷反应元件结合蛋白（CREB）的免疫反应性。在年轻动物中，强烈的CREB免疫反应性局限于细胞核，而不在SNB运动神经元的核仁中。在老年动物中，SNB运动神经元细胞核中CREB免疫反应性的强度和CREB免疫反应性细胞核的数量均显著降低。老年SNB运动神经元中CREB表达的显著下降表明，随着年龄的增长，SNB系统中cAMP介导的基因表达调控发生了改变。
