Matsumoto A, Prins G S
Department of Anatomy, Juntendo University School of Medicine, Hongo, Tokyo, Japan.
Neurosci Lett. 1998 Feb 27;243(1-3):29-32. doi: 10.1016/s0304-3940(98)00078-0.
Androgen receptor (AR) immunoreactivity was examined in androgen-sensitive motoneurons of the spinal nucleus of the bulbocavernosus (SNB) in young and old male rats by immunohistochemistry using the polyclonal antibody, PG21. In young animals, intense AR immunoreactivity was confined to the cell nucleus, but not in the nucleolus of SNB motoneurons. In old animals, both the intensity of AR immunoreactivity in the nuclei and number of AR immunoreactive nuclei of the SNB motoneurons were significantly reduced. Plasma levels of testosterone in old animals were significantly smaller than those in young ones. Age-dependent changes both in AR expression of SNB motoneurons and plasma levels of androgen seem to correlate with the aging of the SNB system.
采用多克隆抗体PG21，通过免疫组织化学方法检测了年轻和老年雄性大鼠球海绵体肌脊髓核（SNB）中雄激素敏感运动神经元的雄激素受体（AR）免疫反应性。在年轻动物中，强烈的AR免疫反应性局限于细胞核，而不在SNB运动神经元的核仁中。在老年动物中，SNB运动神经元细胞核中AR免疫反应性的强度和AR免疫反应性细胞核的数量均显著降低。老年动物的血浆睾酮水平明显低于年轻动物。SNB运动神经元AR表达和雄激素血浆水平的年龄依赖性变化似乎与SNB系统的衰老有关。
