牛18号染色体18q24上载脂蛋白E-C1-C2基因簇的进化保守性

Evolutionary conservation of the apolipoprotein E-C1-C2 gene cluster on bovine chromosome 18q24.

作者信息

Brzozowska A, Sundvold H, Lien S, Rogne S

机构信息

Department of Animal Science, Agricultural University of Norway, As.

出版信息

Gene. 2000 Jan 11;241(2):241-5. doi: 10.1016/s0378-1119(99)00492-8.

DOI:10.1016/s0378-1119(99)00492-8

PMID:10675035

Abstract

We have constructed a long-range restriction map spanning about 250 kb on bovine chromosome 18q24. Our results show that the apolipoprotein C2 (APOC2) gene is located about 25 kb from the APOE gene. Four putative CpG islands are also indicated in the map. Interestingly, a minisatellite located in the third intron of the human and mouse APOC2 genes was also found at identical position in the bovine gene and revealed high sequence identity comparing with the two corresponding sequences. By means of cosmid mapping, we further demonstrate that the APOE-APOC1-APOC2 gene cluster is evolutionary conserved in cattle.

摘要

我们构建了一个跨越牛18号染色体q24约250 kb的长程限制性图谱。我们的结果表明，载脂蛋白C2（APOC2）基因位于距载脂蛋白E（APOE）基因约25 kb处。图谱中还显示了四个推定的CpG岛。有趣的是，在牛基因的相同位置也发现了位于人类和小鼠APOC2基因第三内含子中的一个小卫星，并且与两个相应序列相比显示出高度的序列同一性。通过黏粒图谱分析，我们进一步证明了APOE-APOC1-APOC2基因簇在牛中是进化保守的。