载脂蛋白C2连锁（Acl）基因：小鼠载脂蛋白e-c1-c2基因簇内的一个新基因。

The apolipoprotein C2-linked (Acl) gene: a new gene within the mouse apolipoprotein e-c1-c2 gene cluster.

作者信息

van Eck M M, Hoffer M J, Havekes L M, Frants R R, Hofker M H

机构信息

MGC-Department of Human Genetics, Leiden University, The Netherlands.

出版信息

Genomics. 1994 May 1;21(1):110-5. doi: 10.1006/geno.1994.1231.

DOI:10.1006/geno.1994.1231

PMID:8088778

Abstract

The apolipoprotein E, C1, and C2 genes are contained within a gene cluster in man. Previously, we have shown that this gene cluster has a similar structure in mouse. During the characterization of the mouse Apoc2 gene, evolutionarily conserved and transcribed sequences were found 5' of the Apoc2 gene. In this study, we have shown that these 5' sequences represent a novel gene within the gene cluster designated the apolipoprotein C2-linked gene (Acl). The Acl gene is located 2 kb 5' to the Apoc2 gene. The transcriptional orientation is identical to that of the other genes within the Apoe-c1-c2 gene cluster. We have sequenced the mouse Acl gene at the cDNA and the genomic levels. The gene is composed of three exons spanning a region of approximately 3.6 kb. The Acl gene is expressed in the liver as a transcript 473 bp in size and encodes a putative protein of 124 amino acid residues.

摘要

载脂蛋白E、C1和C2基因位于人类的一个基因簇中。此前，我们已经表明该基因簇在小鼠中具有相似的结构。在对小鼠Apoc2基因进行特征分析的过程中，在Apoc2基因的5'端发现了进化上保守且转录的序列。在本研究中，我们已经表明这些5'端序列代表了该基因簇内的一个新基因，命名为载脂蛋白C2连锁基因（Acl）。Acl基因位于Apoc2基因5'端2 kb处。转录方向与Apoe - c1 - c2基因簇内的其他基因相同。我们已经在cDNA和基因组水平上对小鼠Acl基因进行了测序。该基因由三个外显子组成，跨越约3.6 kb的区域。Acl基因在肝脏中表达，转录本大小为473 bp，编码一个由124个氨基酸残基组成的假定蛋白质。