Shen Y, West C, Hutchins S R
National Risk Management Research Laboratory, Subsurface Protection and Remediation Division, US Environmental Protection Agency, Ada, Oklahoma 74820, USA.
Ecotoxicol Environ Saf. 2000 Jan;45(1):27-32. doi: 10.1006/eesa.1999.1843.
Toluene (methylbenzene) is a common environmental pollutant that is found in many hazardous waste sites and it is an aquifer contaminant. A concern is the potential risk to human and ecosystem health due to exposure to toluene and its major biotransformation products. The cytotoxicity of eight aromatic products of toluene aerobic biotransformation was investigated in bluegill sunfish BF-2 cells. The cytotoxicity was determined using several in vitro assay endpoints. BF-2 cells were propagated at 32 degrees C in an atmosphere of 5% CO2-95% air. The concentrations of these products causing 50% inhibition in cell replication, protein content, uptake of natural red, and colony formation were evaluated and compared. The results of the study indicate a direct relationship between the exposure concentration of these products and observed cytotoxic effects. In descending order of cytotoxicity, the compounds were 3-methylcatechol, 4-methylcatechol, catechol, o-cresol, p-cresol, m-cresol, benzaldehyde, and methyl benzoate.
甲苯（甲基苯）是一种常见的环境污染物，在许多危险废物场地都能找到，并且它还是一种含水层污染物。人们担心的是，接触甲苯及其主要生物转化产物会对人类和生态系统健康构成潜在风险。在蓝鳃太阳鱼BF-2细胞中研究了甲苯有氧生物转化的八种芳香产物的细胞毒性。使用几种体外测定终点来确定细胞毒性。BF-2细胞在32摄氏度、5%二氧化碳-95%空气的气氛中培养。评估并比较了这些产物在细胞复制、蛋白质含量、天然红摄取和集落形成方面引起50%抑制的浓度。研究结果表明这些产物的暴露浓度与观察到的细胞毒性效应之间存在直接关系。按细胞毒性从高到低的顺序，这些化合物依次为3-甲基邻苯二酚、4-甲基邻苯二酚、邻苯二酚、邻甲酚、对甲酚、间甲酚、苯甲醛和苯甲酸甲酯。
