Babich H, Borenfreund E
Toxicol Lett. 1987 Apr;36(2):107-16. doi: 10.1016/0378-4274(87)90174-3.
The in vitro cytotoxicities of various polycyclic aromatic hydrocarbons (PAHs) to bluegill sunfish BF-2 cells were determined with the neutral red assay, which was modified by the incorporation of an S-9 microsomal fraction. Whereas the PAHs per se were weakly cytotoxic, the presence of the S-9 fraction in the incubation mixture increased the cytotoxicity of many of the PAHs, presumably due to the formation of active metabolites. In addition, the cytotoxicity of the PAHs (whether in the absence or presence of the S-9 microsomal fraction) was potentiated if the 6 h exposure was at 37, rather than at 26 degrees C.
采用中性红试验测定了多种多环芳烃（PAHs）对蓝鳃太阳鱼BF-2细胞的体外细胞毒性，该试验通过加入S-9微粒体组分进行了改进。尽管PAHs本身的细胞毒性较弱，但孵育混合物中S-9组分的存在增加了许多PAHs的细胞毒性，这可能是由于活性代谢产物的形成。此外，如果6小时的暴露温度为37℃而非26℃，PAHs的细胞毒性（无论有无S-9微粒体组分）都会增强。
