霍乱弧菌溶血素的细胞毒性细胞空泡化活性。
Cytotoxic cell vacuolating activity from Vibrio cholerae hemolysin.
作者信息
Coelho A, Andrade J R, Vicente A C, Dirita V J
机构信息
Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ21944-970, Brazil.
出版信息
Infect Immun. 2000 Mar;68(3):1700-5. doi: 10.1128/IAI.68.3.1700-1705.2000.
Abstract
A Vibrio cholerae cytotoxin, designated VcVac, was found to cause vacuolation in Vero cells. It was originally detected in the pathogenic O1 Amazonia variant of V. cholerae and later shown to be produced in environmental strains and some El Tor strains. Comparison of VcVac production in various strains suggested that hemolysin was responsible for the vacuolating phenotype. Genetic experiments established a firm correlation between vacuolation and hemolysin production. The mammalian cell vacuolating activity of the V. cholerae hemolysin is a new property of this protein and points to a previously unknown type of interaction between V. cholerae and its host.
摘要
一种名为VcVac的霍乱弧菌细胞毒素被发现可导致Vero细胞出现空泡化。它最初在霍乱弧菌致病性O1亚马逊变种中被检测到，后来证明在环境菌株和一些埃尔托生物型菌株中也会产生。对各种菌株中VcVac产生情况的比较表明，溶血素是导致空泡化表型的原因。遗传学实验确定了空泡化与溶血素产生之间的紧密关联。霍乱弧菌溶血素的哺乳动物细胞空泡化活性是该蛋白的一种新特性，表明霍乱弧菌与其宿主之间存在一种此前未知的相互作用类型。
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