Hong Y, Suzuki S, Yatoh S, Mizutani M, Nakajima T, Bannai S, Sato H, Soma M, Okuda Y, Yamada N
Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Ibaraki-ken, Tsukuba, 305, Japan.
Biochem Biophys Res Commun. 2000 Feb 16;268(2):329-32. doi: 10.1006/bbrc.2000.2140.
It has not been clarified yet as to whether hypoxia and inflammation affect NO synthesis. In this study, we investigated the transcription of inducible nitric oxide synthase (iNOS) mRNA and the production of nitric oxide (NO) in rat smooth muscle cells (SMCs) cultured under hypoxic conditions in the presence and absence of proinflammatory cytokine interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS). We found that hypoxia inhibited the production of NO but did not affect the transcription of iNOS mRNA in rat SMCs treated with IFN-gamma, LPS, or both. These results indicate that O(2) is involved in the regulation of NO synthesis in inflammatory tissues.
关于缺氧和炎症是否影响一氧化氮（NO）的合成，目前尚未明确。在本研究中，我们研究了在存在和不存在促炎细胞因子干扰素-γ（IFN-γ）和脂多糖（LPS）的情况下，在缺氧条件下培养的大鼠平滑肌细胞（SMC）中诱导型一氧化氮合酶（iNOS）mRNA的转录和一氧化氮（NO）的产生。我们发现，缺氧抑制了用IFN-γ、LPS或两者处理的大鼠SMC中NO的产生，但不影响iNOS mRNA的转录。这些结果表明，O(2)参与了炎症组织中NO合成的调节。
