Tarès S, Bergé J B, Amichot M
INRA-IFR 38 "Réponse des organismes aux stress physico-chimiques et biologiques,", Unité Santé Végétale et Environnement, 123 boulevard Francis Meilland, Antibes Cedex, 06606, France.
Biochem Biophys Res Commun. 2000 Feb 24;268(3):677-82. doi: 10.1006/bbrc.2000.2199.
Cytochrome P450 partial sequences were isolated by PCR using genomic DNA from two hymenopteran insects of agronomical importance, Trichogramma cacoeciae, a parasitoid wasp, and Apis mellifera, the honeybee. Four new P450 genes were identified: one honeybee gene belongs to the CYP4 family and was named CYP4G11; the three other genes were from Trichogramma and belong to the CYP4 family (CYP4G12) and to a novel family, the CYP48 one (CYP48A1 and CYP48A2). The four genes contain a short intron (72-95 bp) at the same position as already described for other insect species. The two genes CYP48A1 and CYP48A2 have a supernumary intron (57-71 bp) upstream the first one. Only the two CYP4 genes were constitutively transcribed, at a high level for CYP4G12 and at a low level for CYP4G11. No expression was observed for CYP48A1 and CYP48A2.
利用来自两种具有农业重要性的膜翅目昆虫（寄生蜂小菜蛾赤眼蜂和蜜蜂意大利蜜蜂）的基因组DNA，通过聚合酶链式反应（PCR）分离出细胞色素P450部分序列。鉴定出四个新的P450基因：一个蜜蜂基因属于CYP4家族，被命名为CYP4G11；其他三个基因来自小菜蛾赤眼蜂，属于CYP4家族（CYP4G12）和一个新的家族CYP48（CYP48A1和CYP48A2）。这四个基因在与其他昆虫物种已描述的相同位置含有一个短内含子（72 - 95碱基对）。CYP48A1和CYP48A2这两个基因在第一个内含子上游还有一个额外的内含子（57 - 71碱基对）。只有两个CYP4基因组成型转录，CYP4G12转录水平高，CYP4G11转录水平低。未观察到CYP48A1和CYP48A2的表达。
