Uchio Y, Ochi M, Matsusaki M, Kurioka H, Katsube K
Department of Orthopaedics, Shimane Medical University, 89-1, Enya-Cho, Izumo-Shi, Shimane-Ken 693-8501, Japan.
J Biomed Mater Res. 2000 May;50(2):138-43. doi: 10.1002/(sici)1097-4636(200005)50:2<138::aid-jbm7>3.0.co;2-k.
To evaluate the potential of Atelocollagen gel as a carrier for chondrocyte transplantation, histological and biochemical characteristics of the chondrocytes in gel culture were compared with those in conventional monolayer cultures. Articular chondrocytes from 20 patients were isolated by enzyme digestion, embedded in Atelocollagen gel, and cultured for up to 4 weeks. The effects on proliferation, morphological changes, and synthesis of proteoglycans were analyzed by cell counts, light and electron microscopy, and measurement of isomers of chondroitin sulfates. Chondrocytes embedded in the Atelocollagen gel gradually proliferated and produced chondroitin 6-sulfate, maintaining the chondrocyte phenotype for up to 4 weeks. In contrast, although monolayer chondrocytes increased in number, most could be characterized as being fibroblast-like cells with a reduced capability of producing chondroitin 6-sulfate. The results suggest that Atelocollagen gel permitted a gradual proliferation and matrix synthesis of chondrocytes and maintaining its phenotype. Atelocollagen gel represents an important carrier for the clinical application of cultured chondrocyte transplantation for repair of cartilage defects.
为评估去端肽胶原蛋白凝胶作为软骨细胞移植载体的潜力，将凝胶培养中软骨细胞的组织学和生化特性与传统单层培养中的进行比较。通过酶消化从20名患者中分离出关节软骨细胞，包埋于去端肽胶原蛋白凝胶中，并培养长达4周。通过细胞计数、光镜和电镜以及硫酸软骨素异构体的测量，分析其对增殖、形态变化和蛋白聚糖合成的影响。包埋于去端肽胶原蛋白凝胶中的软骨细胞逐渐增殖并产生6-硫酸软骨素，长达4周维持软骨细胞表型。相比之下，尽管单层软骨细胞数量增加，但大多数可被表征为成纤维细胞样细胞，产生6-硫酸软骨素的能力降低。结果表明，去端肽胶原蛋白凝胶可使软骨细胞逐渐增殖和基质合成，并维持其表型。去端肽胶原蛋白凝胶是用于软骨缺损修复的培养软骨细胞移植临床应用的重要载体。
