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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Matsusaki M, Ochi M, Uchio Y, Shu N, Kurioka H, Kawasaki K, Adachi N

Department of Orthopaedics, Shimane Medical University, Japan.

Gen Pharmacol. 1998 Nov;31(5):759-64. doi: 10.1016/s0306-3623(98)00105-0.

Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5+/-2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3+/-1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3+/-2.1 x 10(5)), 0.1 ng/ ml (11.7+/-2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8+/-2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF.

研究了碱性成纤维细胞生长因子（bFGF）在凝胶培养中能否刺激软骨细胞增殖并合成硫酸软骨素，同时维持其表型。2. 培养3周时，1.0 ng/ml（18.5±2.1×10⁵）和10.0 ng/ml的bFGF组细胞数量（15.3±1.9×10⁵）显著多于0 ng/ml（12.3±2.1×10⁵）、0.1 ng/ml（11.7±2.2×10⁵）和100.0 ng/ml的bFGF组（9.8±2.3×10⁵）。3. 本研究中使用的所有剂量的bFGF均抑制硫酸软骨素6-硫酸酯的合成。4. 即使有bFGF刺激，凝胶培养中的软骨细胞表型也能维持4周。

Matsusaki M, Ochi M, Uchio Y, Shu N, Kurioka H, Kawasaki K, Adachi N

Department of Orthopaedics, Shimane Medical University, Japan.

Gen Pharmacol. 1998 Nov;31(5):759-64. doi: 10.1016/s0306-3623(98)00105-0.

Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5+/-2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3+/-1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3+/-2.1 x 10(5)), 0.1 ng/ ml (11.7+/-2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8+/-2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF.

研究了碱性成纤维细胞生长因子（bFGF）在凝胶培养中能否刺激软骨细胞增殖并合成硫酸软骨素，同时维持其表型。2. 培养3周时，1.0 ng/ml（18.5±2.1×10⁵）和10.0 ng/ml的bFGF组细胞数量（15.3±1.9×10⁵）显著多于0 ng/ml（12.3±2.1×10⁵）、0.1 ng/ml（11.7±2.2×10⁵）和100.0 ng/ml的bFGF组（9.8±2.3×10⁵）。3. 本研究中使用的所有剂量的bFGF均抑制硫酸软骨素6-硫酸酯的合成。4. 即使有bFGF刺激，凝胶培养中的软骨细胞表型也能维持4周。