Hu Y, Chen C, Zhou S
Department of Ophthalmology, Affiliated Xinhua Hospital, Shanghai Second Medical University.
Zhonghua Yan Ke Za Zhi. 1997 Nov;33(6):457-9.
To investigate the inhibitory activity and the effective concentration of daunomycin against the proliferation of lens epithelial cells in vitro, and make progress in pharmacological prevention of posterior capsule opacification.
Cultured bovine, rabbit and human lens epithelial cells were exposed to 0.5, 2.5, 5.0, 7.5 and 10.0 micrograms/ml daunomycin solution for ten minutes. Growth of cells was observed, after 48 hours of exposure, absorbency of the cells was tested by using Giemsa staining and colorimetry, and the median lethal dose (LD50) was calculated respectively.
Daunomycin significantly suppressed the proliferation of bovine, rabbit and human lens epithelial cells in vitro, showing dose-dependent manner. Daunomycin can effectively inhibit the proliferation of human lens epithelial cells at the concentration of 0.5 microgram/ml, almost reaching the greatest effect at the concentration of 7.5 micrograms/ml. The calculated LD50 for bovine, rabbit and human lens epithelial cells was 0.49, 4.30 and 4.06 micrograms/ml respectively.
Short time exposure of daunomycin at low concentration can effectively inhibit the proliferation of lens epithelial cells in vitro, after further research in vivo, daunomycin may possibly become a valuable agent for the prevention of posterior capsule opacification.
研究柔红霉素对体外培养的晶状体上皮细胞增殖的抑制活性及有效浓度，为后囊膜混浊的药物预防研究取得进展。
将培养的牛、兔和人晶状体上皮细胞暴露于0.5、2.5、5.0、7.5和10.0微克/毫升的柔红霉素溶液中10分钟。观察细胞生长情况，暴露48小时后，采用吉姆萨染色和比色法检测细胞吸光度，并分别计算半数致死剂量（LD50）。
柔红霉素在体外显著抑制牛、兔和人晶状体上皮细胞的增殖，呈剂量依赖性。柔红霉素在0.5微克/毫升浓度时可有效抑制人晶状体上皮细胞增殖，在7.5微克/毫升浓度时几乎达到最大抑制效果。计算得出牛、兔和人晶状体上皮细胞的LD50分别为0.49、4.30和4.06微克/毫升。
低浓度柔红霉素短时间暴露可有效抑制体外培养的晶状体上皮细胞增殖，经进一步体内研究后，柔红霉素可能成为预防后囊膜混浊的一种有价值的药物。
