Li Qiu-ming, Wang Meng-hua, Zheng Guang-ying
Department of Ophthalmology, First Clinical Medical College, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Yan Ke Za Zhi. 2006 Jan;42(1):48-53.
To establish an in vitro model of posterior capsule opacification (PCO) by culturing the posterior capsule of bovine lens, to observe the proliferation and differentiation of lens epithelial cells and to study the influence of serum and pranoprofen eyedrops on cell confluence of this model.
The bovine lens posterior capsule was spread on the surface of a 25 ml culture flask with cell layer upward. DMEM with 0%, 10% and 20% fetal calf serum was used as culture medium. The cell coverage and confluence time on the posterior capsule were observed by inverted microscope and the cell morphology was observed by Giemsa staining and scanning electron-microscope. Pranoprofen was added to the culture medium at a concentration similar to the aqueous humor concentration (0.23 mg/L), which was presented at 4 hours after the instillation of pranoprofen eyedrops. The difference of confluence time between the treated group and the control group was compared.
The lens epithelial cells migrated and proliferated rapidly on the posterior capsule from the equatorial region to the center. The cell coverage was increased and the confluence time was shortened with the increase of serum concentration (P < 0.05). The PCO and wrinkles were presented. Pranoprofen at 0.23 mg/L could inhibit the confluence of lens epithelial cells (P < 0.01).
The in vitro model for PCO was an useful method to study the mechanism of PCO formation. Pranoprofen can inhibit the proliferation of lens epithelial cells and is a safe and efficient drug for preventing the occurrence of PCO and can be used as a routine medication after the cataract operation.
通过培养牛晶状体后囊膜建立后囊膜混浊（PCO）的体外模型，观察晶状体上皮细胞的增殖和分化，研究血清和普拉洛芬滴眼液对该模型细胞汇合率的影响。
将牛晶状体后囊膜平铺于25 ml培养瓶表面，细胞层向上。分别用含0%、10%和20%胎牛血清的DMEM作为培养基。通过倒置显微镜观察后囊膜上的细胞覆盖情况和汇合时间，并用吉姆萨染色和扫描电子显微镜观察细胞形态。以与房水浓度相似的浓度（0.23 mg/L）将普拉洛芬加入培养基中，在滴入普拉洛芬滴眼液4小时后加入。比较处理组和对照组汇合时间的差异。
晶状体上皮细胞在赤道区向后囊膜中心迅速迁移和增殖。随着血清浓度的增加，细胞覆盖面积增大，汇合时间缩短（P < 0.05）。出现了PCO和皱纹。0.23 mg/L的普拉洛芬可抑制晶状体上皮细胞的汇合（P < 0.01）。
PCO体外模型是研究PCO形成机制的一种有用方法。普拉洛芬可抑制晶状体上皮细胞的增殖，并是预防PCO发生的一种安全有效的药物，可作为白内障手术后的常规用药。
