Davis L, Kuttan G
Amala Cancer Research Centre, Amala Nagar, Kerala, India.
Cancer Lett. 2000 Jan 1;148(1):9-17. doi: 10.1016/s0304-3835(99)00252-9.
Administration of an extract from the plant Withania somnifera (Family: Solanaceae) (20 mg/dose/animal; i.p.) for five days along with cyclophosphamide (CTX) (1.5 mmol/kg body wt. i.p.) reduced the CTX induced urotoxicity. Morphological analysis of the bladders of the CTX-treated group showed severe inflammation and dark coloration whereas CTX along with the Withania-treated group showed normal bladder morphology. The extract was found to reduce the protein level in the serum (7.92 g/l) after 4 h of CTX treatment, which was higher in the CTX alone-administered group (11.44 g/l). Blood urea N2 level which was drastically enhanced (136.78 mg/100 ml) 2 after the CTX treatment was significantly reduced (52.08 mg/100 ml) when the animals were treated with Withania extract. Similarly the glutathione (GSH) content in both bladder (1.55 micromol/mg protein) and liver (3.76 micromol/mg protein) was enhanced significantly (P<0.001) in the Withania-treated group compared with the CTX alone-treated animals (bladder 0.5 micromol/mg protein; liver 1.2 micromol/mg protein) Histopathological analysis of the bladder of CTX alone-treated group showed severe necrotic damage where as the Withania somnifera-treated group showed normal bladder architecture.
连续五天给动物腹腔注射睡茄（茄科）提取物（20毫克/剂量/动物），同时腹腔注射环磷酰胺（CTX，1.5毫摩尔/千克体重），可减轻CTX诱导的尿毒性。CTX处理组膀胱的形态学分析显示严重炎症和颜色变深，而CTX与睡茄处理组的膀胱形态正常。发现该提取物在CTX处理4小时后可降低血清中的蛋白质水平（7.92克/升），在单独给予CTX的组中该水平较高（11.44克/升）。CTX处理后急剧升高（136.78毫克/100毫升）的血尿素氮水平，在用睡茄提取物处理动物后显著降低（52.08毫克/100毫升）。同样，与单独用CTX处理的动物相比（膀胱0.5微摩尔/毫克蛋白质；肝脏1.2微摩尔/毫克蛋白质），睡茄处理组膀胱（1.55微摩尔/毫克蛋白质）和肝脏（3.76微摩尔/毫克蛋白质）中的谷胱甘肽（GSH）含量均显著提高（P<0.001）。单独用CTX处理组膀胱的组织病理学分析显示严重的坏死损伤，而睡茄处理组的膀胱结构正常。
