Murali Vishnu Priya, Kuttan Girija
Amala Cancer Research Centre, Affiliated to the University of Calicut, Thrissur, Kerala, India.
Amala Cancer Research Centre, Affiliated to the University of Calicut, Thrissur, Kerala, India
Integr Cancer Ther. 2016 Jun;15(2):205-15. doi: 10.1177/1534735415607319. Epub 2015 Sep 29.
Background Curculigo orchioides Gaertn is an ancient medicinal plant (Family: Amaryllidaceae), well known for its immunomodulatory and rejuvenating effects. Cyclophosphamide (CPA) is an alkylating agent widely used for treating a variety of human malignancies, but associated with different toxicities too. Our previous reports regarding the hemoprotective and hepatoprotective effects of the plant against CPA toxicities provide the background for the present study, which is designed to analyze the ameliorative effect of the methanolic extract of C orchioides on the urotoxicity and nephrotoxicity induced by CPA. Methods CPA was administered to male Swiss albino mice at a single dose of 1.5 mmol/kg body weight to induce urotoxicity after 5 days of prophylactic treatment with C orchioides extract (20 mg/kg body weight). Mesna (2-mercaptoethanesulfonate) was used as a control drug. Serum, tissue, and urine levels of kidney function markers and antioxidant levels were checked along with the serum cytokine levels. Results The plant extract was found to be effective in ameliorating the urotoxic and nephrotoxic side effects of CPA. Upregulation of serum interferon-γ and interleukin-2 levels were observed with C orchioides treatment, which was decreased by CPA administration. Besides these, serum tumor necrosis factor-α level was also downregulated by C orchioides treatment. Conclusion Curculigo orchioides was found to be effective against the CPA-induced bladder and renal toxicities by its antioxidant capability and also by regulating the pro-inflammatory cytokine levels.
仙茅是一种古老的药用植物（科：石蒜科），以其免疫调节和恢复活力的作用而闻名。环磷酰胺（CPA）是一种烷基化剂，广泛用于治疗多种人类恶性肿瘤，但也伴有不同的毒性。我们之前关于该植物对CPA毒性的血液保护和肝脏保护作用的报告为当前研究提供了背景，本研究旨在分析仙茅甲醇提取物对CPA诱导的尿毒性和肾毒性的改善作用。方法：在用仙茅提取物（20mg/kg体重）进行5天预防性治疗后，以1.5mmol/kg体重的单剂量给雄性瑞士白化小鼠施用CPA以诱导尿毒性。美司钠（2-巯基乙烷磺酸盐）用作对照药物。检查肾功能标志物的血清、组织和尿液水平以及抗氧化剂水平以及血清细胞因子水平。结果：发现该植物提取物可有效改善CPA的尿毒性和肾毒性副作用。用仙茅治疗观察到血清干扰素-γ和白细胞介素-2水平上调，而CPA给药则使其降低。除此之外，仙茅治疗还下调了血清肿瘤坏死因子-α水平。结论：发现仙茅通过其抗氧化能力以及调节促炎细胞因子水平，对CPA诱导的膀胱和肾脏毒性有效。
