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Dot-blot nitrocellulose enzyme immunoassays for the detection of white-spot virus and yellow-head virus of penaeid shrimp.

作者信息

Nadala E C, Loh P C

机构信息

Department of Microbiology, University of Hawaii, Honolulu 96822, USA.

出版信息

J Virol Methods. 2000 Feb;84(2):175-9. doi: 10.1016/s0166-0934(99)00140-8.

DOI:10.1016/s0166-0934(99)00140-8
PMID:10680967
Abstract

Dot-blot nitrocellulose enzyme immunoassays (DB-NC-EIA) were developed for the detection of white-spot virus (WSV) and yellow-head virus (YHV) in infected shrimp. The assays utilized HRP-conjugated virus-specific antibodies to detect virus antigen present in gill homogenates of infected shrimp spotted onto nitrocellulose membrane. The assays are by far the simplest and most rapid detection methods available for WSV and YHV.

摘要

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