Landry M L, Stanat S, Biron K, Brambilla D, Britt W, Jokela J, Chou S, Drew W L, Erice A, Gilliam B, Lurain N, Manischewitz J, Miner R, Nokta M, Reichelderfer P, Spector S, Weinberg A, Yen-Lieberman B, Crumpacker C
Yale University, New Haven, Connecticut, USA.
Antimicrob Agents Chemother. 2000 Mar;44(3):688-92. doi: 10.1128/AAC.44.3.688-692.2000.
Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cytomegalovirus (CMV) cell-associated clinical isolates. Four characterized and plaque-purified CMV strains, as well as six coded clinical isolates obtained after antiviral therapy, were distributed and tested. Good agreement was obtained for four of the clinical isolates, but a broad distribution of results was obtained for two isolates. Analysis of these results indicates the problems associated with clinical isolates, including the large genetic variability and the highly cell-associated phenotype. This collaborative effort, by addressing these problems, represents a significant step toward the development of a standardized assay.
十二个实验室合作制定并测试了一种针对巨细胞病毒（CMV）细胞相关临床分离株的标准化蚀斑减少试验。分发并测试了四种经过鉴定和蚀斑纯化的CMV毒株，以及六种抗病毒治疗后获得的编码临床分离株。四种临床分离株的结果一致性良好，但两种分离株的结果分布较广。对这些结果的分析表明了与临床分离株相关的问题，包括巨大的基因变异性和高度细胞相关的表型。通过解决这些问题，这项合作努力代表了朝着开发标准化试验迈出的重要一步。
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